Amperometric Detection of <i>Bacillus anthracis</i> Spores: A Portable, Low-Cost Approach to the ELISA

<table>Linear range of PHAD compared to ELISA. PHAD has a dynamic range between 10<sup>3</sup> and <svg height="16.012501" id="M6" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 48.474998 16.012501" width="48.474998" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> cfu/mL, whereas ELISA has a dynamic range between 10<sup>4</sup> and 10<sup>5</sup> cfu/mL. Spore concentrations that produce statistically saturated signals for PHAD (<svg height="16.012501" id="M7" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 48.474998 16.012501" width="48.474998" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,15.738)"><path d="M153 550l-26 -186q79 31 111 31q90 0 141.5 -51t51.5 -119q0 -93 -89 -166q-85 -69 -173 -71q-32 0 -61.5 11.5t-41.5 23.5q-18 17 -17 34q2 16 22 33q14 9 26 -1q61 -50 124 -50q60 0 93 43.5t33 104.5q0 69 -41.5 110t-121.5 41q-53 0 -102 -20l38 305h286l6 -8
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</svg>) and ELISA (10<sup>6</sup>) are indicated. Average signal for <i>Bacillus thuringiensis</i> (<i>Bt</i>, tested at 10<sup>7</sup> cfu/mL) is also shown adjacent to 10<sup>3</sup> values for comparison. Error bars = standard deviation, dotted line = linear range.</table>

International Journal of Electrochemistry

fig2

Figure 2

Figure 2: Amperometric Detection of <i>Bacillus anthracis</i> Spores: A Portable, Low-Cost Approach to the ELISA 

International Journal of Electrochemistry

Amperometric Detection of Bacillus anthracis Spores: A Portable, Low-Cost Approach to the ELISA

Figure 2