International Journal of Forestry Research

International Journal of Forestry Research / 2012 / Article

Research Article | Open Access

Volume 2012 |Article ID 572903 |

Norul Hisham Hamid, Othman Sulaiman, Azmy Mohammad, Norasikin Ahmad Ludin, "The Decay Resistance and Hyphae Penetration of Bamboo Gigantochloa scortechinii Decayed by White and Brown Rot Fungi", International Journal of Forestry Research, vol. 2012, Article ID 572903, 5 pages, 2012.

The Decay Resistance and Hyphae Penetration of Bamboo Gigantochloa scortechinii Decayed by White and Brown Rot Fungi

Academic Editor: Piermaria Corona
Received07 Aug 2012
Accepted13 Sep 2012
Published09 Oct 2012


The decay resistance and hyphae penetration of bamboo Gigantochloa scortechinii decayed by white and brown rot fungi were investigated using scanning electron microscope (SEM). The bamboo grown in natural stand from three different age classes of 0.5, 3.5, and 6.5 years was harvested, oven dried, sterilised, and exposed to agar media containing 4% malt extract and 2% agar technical (no. 3) under laboratory condition for 8 weeks. The deterioration was expressed as percentage of weight loss, and the decay resistance classes were measured according to ASTM D 2017-81 (1986). This study found that the percentage weight loss was greatly reduced with the bamboo ageing. Regardless of age, the G. scortechinii was classified as highly resistant to decay by white and brown rot fungi. The scanning electron microscope (SEM) observation showed that the fungi hyphae mostly penetrated into the vessel and proceeded to the pit and parenchyma cells.

1. Introduction

Bamboos are one of the natural bioresources available in tropic and subtropic countries. They are considered as a renewable plant because the new shoot will be emerged from soil and grows to become mature bamboo replacing any former harvested culm used for utilisation [1]. Bamboo has attracted much attention due to its short maturation age compared to wood. The age influenced the properties and utilisation of bamboo [2]. The relation between bamboo ageing and maturation was discussed by [310]. These researchers made a general agreement that bamboo culm matures at two to three years, reaching its maximum strength. In Guadua angustifolia, [11] found that the bamboo reached a maximum strength and remained almost constant at the age between three to four years. The characterisation of Gigantochloa scortechinii in relation to its anatomical, physical, and chemical properties by [12], suggested that the bamboo culms can be classified as growing, development, and maturation phases at age of 0.5, 1.5, and 3.5 years, respectively. The G. scortechinii aged 3.5 years found to be suitable for any purposes. However this general agreement did not include the decay resistance.

The biological resistance is an important factor for quality control during bamboo processing and utilisation, as the bamboo is considered as susceptible to fungi and insect attacks [13]. In the laboratory test, [14] found that the weight loss of Indonesian bamboo decayed by white rot, brown rot, and soft rot fungi was not significantly different by the culm portions either bottom, middle, or top. However in the further study, [15] found that the bamboo culm aged 6 months decayed more than the older but there were no significant difference between bamboo aged 1 and 3 years. However, these studies did not report the mode of attack by fungi in the bamboo culm.

The G. Scortechinii is the most abundant bamboo species in Peninsular Malaysia, and it was estimated that the Peninsular Malaysia itself had about 1.4 billion bamboo culms (calculated in 6 meter length, [16]). This abundant bamboo resource has a great potential to be exported to other countries as a commodity; therefore, the assessment of its decay resistance is important. The investigation of hyphae penetration in decayed bamboo using scanning electron microscope (SEM) is very important to understand the mode of fungus attack, and the information is useful in terms of selecting the preservation methods and biodegradation control during material storage and processing.

2. Materials and Methods

2.1. Sources of Materials

G. scortechinii, ages ranging from 0.5, 3.5, and 6.5 years old, grown in natural stand in Nami, Kedah (northern part of Peninsular Malaysia) was selected. The age of culms was recognized from the emerging shoot that first came out on the first day. From one clump (one culm per age), they were harvested and immediately coated with wax before being transferred to the laboratory. The basic characteristics of the culms are given in Table 1.

Basic characteristicAge (years)

Internode length (cm)32.935.240
Internode diameter (cm)14.710.516
Culm wall thickness (mm)877

2.2. Preparation of Specimens

Only the sixth internode above ground level was used, and this is considered as diameter at breast height. The culm was cut to disc size of 3 cm thickness and trimmed to the final size of 1 cm. The green density and chemical contents were determined by using the water displacement method and the following standards (Table 2).


Alcohol/toluene extractiveTAPPI T6m-59 [17]
-celluloseTAPPI T9m-54 [17]
LigninTAPPI T13m-54 [17]
AshTAPPI T211om-85 [17]
SilicaTAPPI T244os-77 [17]

The specimens were oven dried at °C, weighed, and sterilized with propylene oxide for two days prior to exposure to white rot (Coriolus versicolor L. ex Fr.) culture and brown rot (Coniophora puteana Scum. ex Fr.) growing on agar media in Petri dishes. The propylene oxide (30% concentration), used due to its inflammability, can completely evaporate from the specimen after treatment and was found suitable to sterilize bamboo. A 4% malt extract and 2% agar technical (no. 3) medium were used for these two basidiomycetes and incubated at 22°C (65–70% relative humidity) for eight weeks.

2.3. Determination of Deterioration

After eight weeks of exposure to fungi, the adhering mycelium was cleaned softly with a brush, and the specimens were weighed and then dried to measure their loss in dry weight by comparing it with the preexposure value. The decay resistance class was determined according to ASTM D2017-81 [20] as shown in Table 3. The average percentage weight loss is indicated by the resistance classes to specific test fungi that ranged from highly resistant to slightly resistant or nonresistant.

Average weight loss (%)Resistance class

0 to 10Highly resistant
11 to 24Resistant
25 to 44Moderately resistant
45 or aboveSlightly resistant or nonresistant

2.4. Scanning Electron Microscope (SEM) Observation

Specimen exposed to fungi was sliced and fixed with glutaraldehyde solution (5%), ringed with phosphate buffer (ph 7.2), and osmium tetraoxide solution (1%) in accordance with that described by [2123].

3. Results and Discussion

3.1. Weight Loss

The percentage weight loss of bamboo culms at different age groups against decay fungi in relation to the decay resistance classes is given in Table 4. The percentage weight losses were differed between the culm age and the decay fungi. In general, the G. scortechinii culms are more susceptible to brown rot than white rot fungi, and the percentage weight losses were ranged between 8.90 to 9.95 and 5.30 to 9.90, respectively. For comparison, the percentage weight losses of Bambusa maculata, G. Atroviolacea, and Phyllostachys pubescens decayed by brown rot (Coniophora puteana 167) after one-year exposure were ranged 2.8 to 4.2, 5.2 to 5.9, and 4.6 to 4.8, respectively [15]. The percentage weight losses of B. vulgaris, Dendrocalamus asper, G. apus, G. atroviolacea, and G. pseudoarundinacea decayed by white rot (Coriolus versicolor FRI Japan-1030) after 12 weeks exposure were 7.2, 15.2, 4.8, 7.7, and 20.7 [14]. This indicates that the variability of decay resistance is mostly influenced by bamboo species and fungus origin. Therefore, decay resistance test for eight-week exposure period of G. Scortechinii used in this study was considered reliable to investigate the effect of bamboo ageing on the decay resistance.

Age (yrs)Percentage weight loss and decay resistance class
White rotResistance classBrown rotResistance class

0.59.90 (3.58)HR9.95 (2.47)HR
3.59.24 (3.65)HR9.49 (1.80)HR
6.55.30 (2.45)HR8.90 (1.86)HR

HR: Highly resistant. The values in parenthesis are standard deviation.

The percentage weight loss of decayed G. scortechinii decreased with ageing. This was clearly seen by a significant difference of percentage weight loss for different bamboo ages (Table 5). The oldest culm (6.5 years) had lowest percentage weight loss, followed by 3.5 years and the youngest ones. All the age groups were highly resistant to white and brown rot fungi. A higher lignin, silica contents as well as density may probably be the three major factors which reduced the deterioration in the oldest culm (Table 6). The incomplete lignification process of fibres and part of the ground parenchyma tissue within the youngest culm (0.5 years) could have attributed to the higher deterioration [24]. The amount of ethanol/toluene extractive content in G. scortechinii which ranged from 5.8% (0.5 years), 5.3% (3.5 years), and 5.6% (6.5 years) did not give any advantage for decay resistance.

FactorsDf value and
statistical significance

Age X fungi23.41**

** , *** , Df: degree of freedom, : distribution.

PropertiesAge (years)

Oven dry density (g/m3)0.53 (0.04)0.61 (0.03)0.68 (0.07)
Alcohole/toluene extractive (%)5.8 (1.5)5.3 (0.4)5.6 (0.2)
Holocellulose (%)78.6 (0.3)80.6 (2.3)82.3 (1.8)
-cellulose (%)64.6 (0.6)64.6 (0.3)64.6 (0.2)
Lignin (%)23.4 (0.3)27.8 (1.9)29.0 (0.4)
Ash (%)1.9 (0.02)2.8 (0.10)3.5 (0.02)
Silica (%)0.6 (0.2)1.7 (0.02)2.0 (0.2)

The values in parenthesis are standard deviation.
3.2. Hyphae Penetration

The cavities formed by white and brown rot fungi as observed through the SEM are shown in Figures 1 to 6. It was found that the hyphae penetrated mostly into the metaxylem vessel rather than the fibre lumen’s cell for both fungi (Figures 1 and 2). It was also observed that, from the lumen, the hyphae further penetrated into the parenchyma cell, where starch was mostly deposited (Figure 3, white rot). Larger space available in metaxylem vessel lumen, generally, may provide better movement for the penetration (Figures 4 and 5, brown rot). The average metaxylem vessel diameters were 0.51 mm (0.5 years), 0.57 mm (3.5 years), and 0.50 mm (6.5 years); meanwhile the average fibre lumen diameters were 10 μm (0.5 years), 8 μm (3.5 years) and 9 μm (6.5 years), respectively [12]. The hyphae also used the pits present in the vessel walls as a pathway to penetrate the neighbouring cells (Figure 6, brown rot).

4. Conclusions

The susceptibility of G. scortechinii to white and brown rot fungi, measure by percentage weight loss, is greatly reduced with the bamboo ageing. The mode of fungi attacks is by penetrating the hyphae into the large metaxylem vessel cell and then proceeds to the parenchyma cell where starch is abundantly deposited. All bamboo culms of different age groups are highly resistant to decay by white and brown rot fungi.


  1. F. Zhou, ,Selected Works of Bamboo Research, Nanjing Forestry University, Nanjing, China, 2000.
  2. W. Liese, “Structural research issue on bamboo. Special Focus,” INBAR, vol. 5, no. 1-2, pp. 27–29, 1997. View at: Google Scholar
  3. F. Zhou, “Studies on physical and mechanical properties of bamboo wooDs,” Journal Nanjing Technology College of Forestry Product, vol. 2, pp. 1–32, 1981. View at: Google Scholar
  4. Z. B. Espiloy, “Physico—mechanical properties and anatomical relationships of some Philippine bamboos,” in Recent Research on Bamboo. Proceedings of An International Workshop, A. N. Rao et al., Ed., pp. 257–264, HangZhou, China, 1987. View at: Google Scholar
  5. Z. B. Espiloy, “Effect of age on the physio—mechanical properties of some Philippine Bamboo,” in Bamboo in Asia and the Pacific. Proceedings of the 4th International Bamboo Workshop, pp. 180–182, FORSPA, Chiangmai, Thailand, November 1994. View at: Google Scholar
  6. E. A. Widjaja and Z. Risyad, “Anatomical properties of some bamboo utilized in Indonesia,” in Recent Research on Bamboo. Proceedings of an International Workshop, A. N. Rao et al., Ed., pp. 224–246, October 1987. View at: Google Scholar
  7. M. Abd Latif, W. A. Wan Tarmeze, and A. Fauzidah, “Anatomical features and mechanical properties of three Malaysian bamboo,” Journal of Tropical Forest Science, vol. 2, pp. 227–234, 1990. View at: Google Scholar
  8. M. A. Sattar, M. F. Kabir, and D. K. Bhattacharjee, “Effect of age and height position of Muli (Melocanna baccifera) and Borak (Bambusa balcooa) bamboos on their physical and mechanical properties,” in Proceedings of the 4th International Workshop on Bamboo in Asia and the Pacific, pp. 183–187, FORSPA, Chiangmai, Thailand, November1991. View at: Google Scholar
  9. W. Liese and G. Weiner, “Ageing of bamboo culms. A review,” Wood Science and Technology, vol. 30, no. 2, pp. 77–89, 1996. View at: Google Scholar
  10. X. B. Li, T. F. Shupe, G. F. Peter, C. Y. Hse, and T. L. Eberhardt, “Chemical changes with maturation of the bamboo species Phyllostachys pubescens,” Journal of Tropical Forest Science, vol. 19, no. 1, pp. 6–12, 2007. View at: Google Scholar
  11. D. J. F. Correal and C. Juliana Arbeláez, “Influence of age and height position on colombian Guadua angustifolia bambo mechanical properties,” Maderas, vol. 12, no. 2, pp. 105–113, 2010. View at: Publisher Site | Google Scholar
  12. H. N. Hisham, S. Othman, H. Rokiah, M. A. Latif, S. Ani, and M. M. Tamizi, “Characterization of bamboo Gigantochloa scortechinii at different ages,” Journal of Tropical Forest Science, vol. 18, no. 4, pp. 236–242, 2006. View at: Google Scholar
  13. W. Liese, “Bamboos-biology, silvics, properties, utilization,” Schriftenreihe Der GTZ no. 180, Deutsche Geselschaft fiir Technische Zusanimenarbeit (GTZ), Eschborn, Germany, 1985. View at: Google Scholar
  14. S. Suprapti, “Decay resistance of five Indonesian bamboo species against fungi,” Journal of Tropical Forest Science, vol. 22, no. 3, pp. 287–294, 2010. View at: Google Scholar
  15. O. Schmidt, O. Wei, O. Ds, W. Liese, and E. Wollenberg, “Fungal degradation of bamboo samples,” Holzforschung, vol. 65, pp. 883–888, 2011. View at: Google Scholar
  16. W. Razak, H. Norul Hisham, W. S. Hisham, W. A. Wan Tarmeze, and M. Mohd Tamizi, Manufacturing Process of Laminated Bamboo (Lamboo), Bamboo Training Course, Pahang, Malaysia, 2001.
  17. Anonymous, “TAPPI official testing procedure,” Technical Association of the Pulp and Paper Industry, Atlanta, Ga, USA, 1978. View at: Google Scholar
  18. L. E. Wise, M. Murphy, and E. E. D'Addieco, “Chlorite holocellulose, its fractionation and bearing on summative wood analysis and on studies on the hemicelluloses,” vol. 122, no. 2, article 35, 1946. View at: Google Scholar
  19. F. R. Humphreys and J. Kelly, “A method for the determination of starch in wood,” Analytica Chimica Acta, vol. 24, pp. 66–70, 1961. View at: Google Scholar
  20. Anonymous, ASTM D2017-81. Accelerated Laboratory Test of Natural Decay Resistance of Woods, 1986.
  21. G. A. Meek, “Electron Microscopic Histology,” in Practical Electron Microscopy For Biologis, vol. 2, pp. 413–485, 1977. View at: Google Scholar
  22. J. R. Barnett, “Microscopy of wood,” Microscopy and Analysis, vol. 4, pp. 11–13, 1988. View at: Google Scholar
  23. Anonymous, SEM Tissue Preparation Method, Electron Microscopic Unit, School of Biology, Universiti Sains Malaysia (USM), Pulau Pinang, Malaysia, 1997.
  24. R. S. Murphy, S. Othman, and K. L. Alvin, “Fungal deterioration of bamboo cell walls,” in The Bamboos, Linnean Sociaty Symposium Series, G. P. Chapman, Ed., pp. 323–332, 1996. View at: Google Scholar

Copyright © 2012 Norul Hisham Hamid et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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