Plant Regeneration through Callus Cultures from Leaf Explant of Sumatra Benzoin (Styrax benzoin)

Table 5

Regeneration of shoots and roots in Styrax benzoin Dryander calli obtained from young leaves after 12 weeks of culture.

Treatments	Shoots		Roots
	Explants with shoots (%)	Average number of shoots/explants	Explants with roots (%)	Average number of roots/explants
A0B0	—	0.00^e	60.12	0.54 ± 0.01^ef
A0B1	59.81	1.18 ± 0.16^d	69.24	0.54 ± 0.01^ef
A0B2	70.06	1.92 ± 0.62^bc	—	0^ef
A0B3	60.22	1.95 ± 1.31^b	—	0^ef
A1B0	—	0.00^e	75.15	2.59 ± 0.05^cd
A1B1	60.12	1.77 ± 1.18^cd	70.25	2.96 ± 0.06^bcd
A1B2	69.76	2.96 ± 0.79^abc	70.06	1.35 ± 0.03^de
A1B3	75.05	3.63 ± 0.54^a	70.05	4.58 ± 0.09^abc
A2B0	—	0.00^e	70.16	2.37 ± 0.05^cd
A2B1	—	0.00^e	70.34	2.96 ± 0.06^bcd
A2B2	69.85	1.59 ± 0.16^bc	70.23	1.35 ± 0.03^de
A2B3	70.18	2.75 ± 0.67^ab	69.65	1.88 ± 0.04^de
A3B0	—	0.00^e	70.38	5.92 ± 0.12^a
A3B1	—	0.00^e	60.05	5.03 ± 0.10^ab
A3B2	59.72	1.08 ± 0.72^e	69.65	2.96 ± 0.06^bcd
A3B3	70.15	2.32 ± 0.20^abc	69.63	1.62 ± 0.03^de
Average	41.56	1.32 ± 0.40	60.31	2.62 ± 0.05

The experiments were conducted with five replicates. Experimental treatments are described in Table 2. Data shown are mean of five replicates followed by different letters are significant according to Duncan’s multiple range analysis ().