Research Article

cpDNA-Gene-Sequence-Based Genetic Diversity, Population Structure, and Gene Flow Analysis of Ethiopian Lowland Bamboo (Bambusinea: Oxytenanthera abyssinica (A. Rich.) Munro)

Table 2

cpDNA primer sequences, their approximate amplified size, and PCR profile.

Primer nameAmplified size (bp)Primer setsAmplification patternPCR profile (all end with 4°C hold)

matK∼ 1,350F: CGTTCTGACCATATTGCACTATG
R: AACTAGTCGGATGGAGTAG
Excellent96°C, 3 min.; 35X (96°C, 30 sec., 53, 30 sec., 72°C 1 min.); 72°C, 20 min
ndhF∼ 1,140F: GTCTCAATTGGGTTATATGATG
R: CCCCCTAYATATTTGATACCTTCTCC
Excellent
rps16∼ 860F: AAACGATGTGGTARAAAGCAAC
R: AACATCWATTGCAASGATTCGATA
Excellent