International Journal of Genomics

International Journal of Genomics / 2000 / Article

Review Article | Open Access

Volume 1 |Article ID 156745 | https://doi.org/10.1155/2000/156745

Albertha J. M. Walhout, Simon J. Boulton, Marc Vidal, "Yeast Two-Hybrid Systems and Protein Interaction Mapping Projects for Yeast and Worm", International Journal of Genomics, vol. 1, Article ID 156745, 7 pages, 2000. https://doi.org/10.1155/2000/156745

Yeast Two-Hybrid Systems and Protein Interaction Mapping Projects for Yeast and Worm

Abstract

The availability of complete genome sequences necessitates the development of standardized functional assays to analyse the tens of thousands of predicted gene products in high-throughput experimental settings. Such approaches are collectively referred to as ‘functional genomics’. One approach to investigate the properties of a proteome of interest is by systematic analysis of protein–protein interactions. So far, the yeast two-hybrid system is the most commonly used method for large-scale, high-throughput identification of potential protein–protein interactions. Here, we discuss several technical features of variants of the two-hybrid systems in light of data recently obtained from different protein interaction mapping projects for the budding yeast Saccharomyces cerevisiae and the nematode Caenorhabditis elegans.

Copyright © 2000 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Related articles

No related content is available yet for this article.
 PDF Download Citation Citation
 Order printed copiesOrder
Views453
Downloads1221
Citations

Related articles

No related content is available yet for this article.

Article of the Year Award: Outstanding research contributions of 2021, as selected by our Chief Editors. Read the winning articles.