The SPOT™ technology for highly parallel synthesis of peptides on flat surfaces in array
type format has evolved into a versatile toolbox for a variety of applications in proteomics
such as mapping protein-protein interactions and profiling the substrate specificity of
enzymes such as kinases and proteases. Originally developed for the synthesis of short
overlapping peptide sequences for mapping antibody epitopes this technology has recently
been extended to the synthesis of functional protein domains. This opens up a variety of
future applications such as target identification and protein expression profiling.