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Research Article | Open Access
Heterologous Array Analysis in Pinaceae: Hybridization of Pinus taeda cDNA Arrays with cDNA from Needles and Embryogenic Cultures of P. taeda, P. sylvestris or Picea abies
Leonel van Zyl,1 Sara von Arnold,2 Peter Bozhkov,2 Yongzhong Chen,2 Ulrika Egertsdotter,3 John MacKay,3 Ronald R. Sederoff,1 Jing Shen,4 Lyubov Zelena,2 and David H. Clapham
21Forest Biotechnology Group, Centennial Campus, Box 7247, North Carolina State University, Raleigh, NC 27695, USA
2Department of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, Uppsala S-750 07, Sweden
3Institute of Paper Science and Technology, Forest Biology Group, 500 10th Street NW, Atlanta, GA 30318, USA
4Bioinformatics Research Center, Centennial Campus, North Carolina State University, Raleigh, NC 27695, USA
Abstract
Hybridization of labelled cDNA from various cell types with high-density arrays of expressed sequence tags is a powerful technique for investigating gene expression. Few conifer cDNA libraries have been sequenced. Because of the high level of sequence
conservation between Pinus and Picea we have investigated the use of arrays from one genus for studies of gene expression in the other. The partial cDNAs from 384 identifiable genes expressed in differentiating xylem of Pinus taeda were printed on nylon membranes in randomized replicates. These were hybridized with labelled cDNA from needles or embryogenic cultures of Pinus taeda, P. sylvestris and Picea abies, and with labelled cDNA from leaves of Nicotiana tabacum. The Spearman correlation of gene expression for pairs of conifer species was high for needles
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Copyright
Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
