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Comparative and Functional Genomics
Volume 3, Issue 4, Pages 362-365
http://dx.doi.org/10.1002/cfg.191
Conference Review

Microarrays for Public Health: Genomic Epidemiology of Tuberculosis

Department of Microbiology and Immunology, Medical Sciences Building, PO Box 138, University Road, Leicester LE1 9HN, UK

Received 31 May 2002; Accepted 12 June 2002

Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In response to a large local school-based outbreak of tuberculosis, we have been evaluating the utility of microarray bacterial genomic analysis in outbreak management. After initial comparison of the isolate from the index case with Mycobacterium tuberculosis H37Rv, it was possible to design robust PCRs directed towards strain-specific deletions. Rapid PCR analysis of isolates proved valuable in determining whether or not other isolates were compatible with the outbreak strain and further microarray studies revealed genetic markers that could be used to discriminate between locally circulating strains.We suggest that this approach forms the basis for developing rapid local genotyping schemes applicable to M. tuberculosis and that application to other pathogens warrants consideration.