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Comparative and Functional Genomics
Volume 4 (2003), Issue 5, Pages 525-530
http://dx.doi.org/10.1002/cfg.326
Conference Review

Padlock and Proximity Probes for In Situ and Array-Based Analyses: Tools for the Post-Genomic Era

Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Sweden

Received 6 June 2003; Revised 5 August 2003; Accepted 5 August 2003

Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Highly specific high-throughput assays will be required to take full advantage of the accumulating information about the macromolecular composition of cells and tissues, in order to characterize biological systems in health and disease. We discuss the general problem of detection specificity and present the approach our group has taken, involving the reformatting of analogue biological information to digital reporter segments of genetic information via a series of DNA ligation assays. The assays enable extensive, coordinated analyses of the numbers and locations of genes, transcripts and protein.