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Comparative and Functional Genomics
Volume 4 (2003), Issue 2, Pages 208-215
http://dx.doi.org/10.1002/cfg.281
Conference review

Global Analysis of Cell Type-Specific Gene Expression

University of Arizona, Department of Plant Sciences, 303 Forbes Building, Tucson, AZ 85721, USA

Received 4 February 2003; Revised 5 February 2003; Accepted 6 February 2003

Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The tissues and organs of multicellular eukaryotes are frequently observed to comprise complex three-dimensional interspersions of different cell types. It is a reasonable assumption that different global patterns of gene expression are found within these different cell types. This review outlines general experimental strategies designed to characterize these global gene expression patterns, based on a combination of methods of transgenic fluorescent protein (FP) expression and targeting, of flow cytometry and sorting and of high-throughput gene expression analysis.