International Journal of Genomics

International Journal of Genomics / 2004 / Article

Short communication | Open Access

Volume 5 |Article ID 569342 | https://doi.org/10.1002/cfg.392

Alexander M. van der Linden, Ronald H. A. Plasterk, "Shotgun Cloning of Transposon Insertions in the Genome of Caenorhabditis elegans", International Journal of Genomics, vol. 5, Article ID 569342, 5 pages, 2004. https://doi.org/10.1002/cfg.392

Shotgun Cloning of Transposon Insertions in the Genome of Caenorhabditis elegans

Received15 Apr 2003
Revised21 Jan 2004
Accepted28 Jan 2004

Abstract

We present a strategy to identify and map large numbers of transposon insertions in the genome of Caenorhabditis elegans. Our approach makes use of the mutator strain mut-7, which has germline-transposition activity of the Tc1/mariner family of transposons, a display protocol to detect new transposon insertions, and the availability of the genomic sequence of C. elegans. From a pilot insertional mutagenesis screen, we have obtained 351 new Tc1 transposons inserted in or near 219 predicted C. elegans genes. The strategy presented provides an approach to isolate insertions of natural transposable elements in many C. elegans genes and to create a large-scale collection of C. elegans mutants.

Copyright © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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