International Journal of Genomics

International Journal of Genomics / 2004 / Article

Conference review | Open Access

Volume 5 |Article ID 941402 | https://doi.org/10.1002/cfg.396

Louisa Matthew, "RNAi for Plant Functional Genomics", International Journal of Genomics, vol. 5, Article ID 941402, 5 pages, 2004. https://doi.org/10.1002/cfg.396

RNAi for Plant Functional Genomics

Received06 Feb 2004
Accepted06 Feb 2004

Abstract

A major challenge in the post-genome era of plant biology is to determine the functions of all the genes in the plant genome. A straightforward approach to this problem is to reduce or knock out expression of a gene with the hope of seeing a phenotype that is suggestive of its function. Insertional mutagenesis is a useful tool for this type of study, but it is limited by gene redundancy, lethal knock-outs, nontagged mutants and the inability to target the inserted element to a specific gene. RNA interference (RNAi) of plant genes, using constructs encoding self-complementary ‘hairpin’ RNA, largely overcomes these problems. RNAi has been used very effectively in Caenorhabditis elegans functional genomics, and resources are currently being developed for the application of RNAi to high-throughput plant functional genomics.

Copyright © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


More related articles

 PDF Download Citation Citation
 Order printed copiesOrder
Views187
Downloads1001
Citations

Related articles

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.