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Comparative and Functional Genomics
Volume 6, Issue 5-6, Pages 301-306
http://dx.doi.org/10.1002/cfg.484
Research article

Characterization of Expressed Sequence Tags From a Gallus gallus Pineal Gland cDNA Library

1Comparative Genomics Lab, Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg 24061, VA, USA
2Division of Cardiology, Department of Medicine, University of Leicester, Leicester, UK
32250 Litton Reaves Hall, Blacksburg 24061, VA, USA

Received 12 January 2005; Revised 22 May 2005; Accepted 1 June 2005

Copyright © 2005 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The pineal gland is the circadian oscillator in the chicken, regulating diverse functions ranging from egg laying to feeding. Here, we describe the isolation and characterization of expressed sequence tags (ESTs) isolated from a chicken pineal gland cDNA library. A total of 192 unique sequences were analysed and submitted to GenBank; 6% of the ESTs matched neither GenBank cDNA sequences nor the newly assembled chicken genomic DNA sequence, three ESTs aligned with sequences designated to be on the Z_random, while one matched a W chromosome sequence and could be useful in cataloguing functionally important genes on this sex chromosome. Additionally, single nucleotide polymorphisms (SNPs) were identified and validated in 10 ESTs that showed 98% or higher sequence similarity to known chicken genes. Here, we have described resources that may be useful in comparative and functional genomic analysis of genes expressed in an important organ, the pineal gland, in a model and agriculturally important organism.