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Comparative and Functional Genomics
Volume 2008, Article ID 254836, 12 pages
http://dx.doi.org/10.1155/2008/254836
Research Article

Effects of Subminimum Inhibitory Concentrations of Antibiotics on the Pasteurella multocida Proteome: A Systems Approach

1College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
2Mississippi State University Institute for Digital Biology, Mississippi State University, Mississippi State, MS 39762, USA
3Mississippi Agriculture and Forestry Experiment Station, Mississippi State University, Mississippi State, MS 39762, USA
4MSU Life Sciences and Biotechnology Institute, Mississippi State University, Mississippi State, MS 39762, USA

Received 6 December 2007; Accepted 19 February 2008

Academic Editor: John Parkinson

Copyright © 2008 Bindu Nanduri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To identify key regulators of subminimum inhibitory concentration (sub-MIC) antibiotic response in the Pasteurella multocida proteome, we applied systems approaches. Using 2D-LC-ESI- , we achieved 53% proteome coverage. To study the differential protein expression in response to sub-MIC antibiotics in the context of protein interaction networks, we inferred P. multocida Pm70 protein interaction network from orthologous proteins. We then overlaid the differential protein expression data onto the P. multocida protein interaction network to study the bacterial response. We identified proteins that could enhance antimicrobial activity. Overall compensatory response to antibiotics was characterized by altered expression of proteins involved in purine metabolism, stress response, and cell envelope permeability.