Global Transcriptional Analysis of Yeast Cell Death Induced by Mutation of Sister Chromatid Cohesin
Figure 6
UV radiation caused increase
of apoptotic cell death in mcd1-1 and pds5-1 mutants. (a) wild type, pds5-1
cells, and mcd1-1 cells were exposed to UV radiation (150 J/) then
incubated for 20 hours at . Cells were stained with DAPI to identify the
fragmented nuclei. Data are from 3 independent experiments; at least 100 cells were counted for each experiment. (b) ROS
was produced in pds5-1 and mcd1-1 mutants. Wild type and the mcd1-1 and
pds5-1mutants were exposed to UV radiation (150 J/) then incubated
for 2 and 18 hours at . Dihydrorhodamine 123 (Priault et al. pages 684–91) was added into cell culture
and incubated for another 2 hours, ROS production was measured using a
microplate reader.
