Constructing Physical and Genomic Maps for Puccinia striiformis f. sp. tritici, the Wheat Stripe Rust Pathogen, by Comparing Its EST Sequences to the Genomic Sequence of P. graminis f. sp. tritici, the Wheat Stem Rust Pathogen

Table 3

Physical groups of Pst corresponding to Pgt supercontigs. Size of each Pgt supercontig and Pst EST coverage in the supercontig, number of contigs, number of ESTs in the urediniospore (Ured), germinated urediniospore (GermUred) and haustoria (Haus) libraries, and total average size of aligned ESTs and Pst gene density in each Pst physical group/Pgt supercontig.

Pst physical		Size (bp)	No. of					Average	Pst gene
		covered	Pst	No. of Pst ESTs in each library and total				size (bp)	density in
group/Pgt	Size (bp)	By Pst	EST					of aligned	supercontig
supercontig	in Pgt	ESTs	contigs	Ured	GermUred	Haus	Total	Pst ESTs	(bp/gene)
1	3,081,398	29,571	35	48	18	87	153	193	20,140
2	2,570,998	18,205	39	30	15	70	115	158	22,357
3	2,616,274	16,097	38	21	20	67	108	149	24,225
4	1,978,325	9,744	33	18	7	61	86	113	23,004
5	2,008,477	15,513	32	25	15	58	98	158	20,495
6	1,808,965	14,784	30	13	10	64	87	170	20,793
7	1,797,936	8,626	25	10	11	32	53	163	33,923
8	1,737,638	10,749	30	4	11	60	75	143	23,169
9	1,714,174	14,977	25	12	13	46	71	211	24,143
10	1,640,743	14,619	25	33	19	42	94	156	17,455
11	1,547,344	12,529	26	12	7	66	85	147	18,204
12	1,543,397	8,518	20	8	4	42	54	158	28,581
13	1,556,540	8,681	23	7	10	36	53	164	29,369
14	1,510,324	12,100	26	16	11	46	73	166	20,689
15	1,374,611	15,098	24	16	10	53	71	213	19,361
16	1,217,956	12,940	22	11	10	49	70	185	17,399
17	1,242,959	18,124	28	25	9	74	109	166	11,403
18	1,195,459	5,965	16	10	6	28	44	136	27,170
19	1,137,327	7,582	13	6	3	49	58	131	19,609
20	1,198,131	9,437	24	9	5	42	56	169	21,395
21	1,084,580	7,679	17	8	6	49	63	122	17,216
22	1,051,806	5,445	17	6	9	13	28	194	37,565
23	1,003,138	10,246	10	8	8	29	45	228	22,292
24	1,008,357	9,373	18	13	8	34	55	170	18,334
25	1,068,291	9,855	18	17	10	34	61	162	17,513
26	1,006,249	10,628	19	8	6	45	59	180	17,055
27	1,005,714	8,119	15	7	5	36	48	169	20,952
28	964,966	6,986	14	10	13	20	43	162	22,441
29	999,150	4,693	16	5	5	24	34	138	29,387
30	919,905	7,064	10	14	7	22	43	164	21,393
31	986,084	6,076	13	7	3	27	37	164	26,651
32	894,979	7,030	16	7	6	40	53	133	16,886
33	889,308	108,494	3	17	8	20	45	2411	19,762
34	856,319	4,895	8	5	4	22	31	158	27,623
35	904,227	9,214	20	16	3	33	52	177	17,389
36	867,522	3,694	10	5	3	11	19	194	45,659
37	820,150	3,615	12	5	0	22	27	134	30,376
38	803,102	3,888	9	8	8	7	23	169	34,917
39	752,863	4,021	11	3	4	28	35	115	21,510
40	667,254	18,530	11	6	8	24	38	488	17,559
41	658,202	4,815	8	11	5	5	21	229	31,343
42	682,257	6,622	10	10	4	26	40	166	17,056
43	654,820	3,637	12	7	4	20	31	117	21,123
44	680,700	4,941	13	2	1	33	36	137	18,908
45	611,533	5,287	9	8	2	30	40	132	15,288
46	603,048	3,148	10	8	5	9	22	143	27,411
47	640,690	3,675	12	9	6	10	25	147	25,628
48	569,981	2,235	7	5	3	15	23	97	24,782
49	609,911	2,601	9	2	3	16	21	124	29,043
50	685,923	5,158	15	2	2	43	47	110	14,594
51	574,326	2,770	9	8	4	14	26	107	22,089
52	572,077	2,928	9	10	1	9	20	146	28,604
53	591,244	2,420	9	2	1	15	18	134	32,847
54	525,265	6,453	8	2	2	23	27	239	19,454
55	542,982	2,301	7	7	3	6	16	144	33,936
56	577,102	1,706	9	5	0	11	16	107	36,069
57	480,201	2,436	5	2	3	16	21	116	22,867
58	496,650	2,974	10	8	4	10	22	135	22,575
59	489,205	5,657	5	4	3	13	20	283	24,460
60	436,003	2,794	4	2	3	13	18	155	24,222
61	469,309	5,578	11	6	6	20	32	174	14,666
62	428,160	8,885	9	30	6	34	70	127	6,117
63	433,102	1,713	7	6	2	7	15	114	28,873
64	440,512	1,559	10	2	2	9	13	120	33,886
65	407,335	1,490	5	1	2	7	10	149	40,734
66	388,993	1,775	6	3	2	8	13	137	29,923
67	403,504	5,231	9	4	2	22	28	187	14,411
68	403,089	2,587	8	6	5	8	19	136	21,215
69	367,522	1,464	5	6	0	2	8	183	45,940
70	386,059	1,545	5	0	1	8	9	172	42,895
71	392,332	2,229	9	0	1	19	20	111	19,617
72	398,881	3,281	12	6	1	15	22	149	18,131
73	360,371	5,159	8	0	1	30	31	166	11,625
74	351,149	2,082	5	2	5	2	9	231	39,017
75	350,882	796	7	2	1	7	10	80	35,088
76	341,344	2,159	4	2	0	14	16	135	21,334
77	304,582	1,463	4	0	2	11	13	113	23,429
78	296,996	2,909	2	5	6	6	17	171	17,470
79	286,933	1,664	3	0	1	9	10	166	28,693
80	312,600	2,919	7	2	0	21	23	127	13,591
81	341,312	1,538	9	4	2	8	13	118	26,255
82	375,963	1,130	5	1	0	6	7	161	53,709
83	301,412	4,474	9	6	2	20	28	160	10,765
84	306,211	2,780	4	6	4	5	15	185	20,414
85	286,187	2,285	5	6	5	2	13	176	22,014
86	268,723	3,915	6	2	2	23	27	145	9,953
87	273,404	1,018	3	0	0	12	12	85	22,784
88	271,488	2,241	4	5	1	7	13	172	20,884
89	281,218	997	4	6	1	1	8	125	35,152
90	282,829	2,792	5	1	2	7	10	279	28,283
91	268,653	2,792	5	4	4	3	11	254	24,423
92	249,303	1,055	4	2	2	2	6	176	41,551
93	254,338	2,955	8	2	2	20	24	123	10,597
94	224,654	1,369	6	3	1	8	12	114	18,721
95	268,031	845	4	3	1	3	7	121	38,290
96	240,035	984	5	2	2	5	9	109	26,671
97	283,953	370	3	2	0	1	3	123	94,651
98	259,162	1,865	4	1	3	6	10	187	25,916
99	245,804	345	4	0	0	5	5	69	49,161
100	212,873	1,355	4	4	1	2	7	194	30,410
101	249,761	566	4	2	0	4	6	94	41,627
102	224,856	1,583	4	3	2	5	10	158	22,486
103	231,854	1,007	5	5	0	4	9	112	25,762
104	230,218	1,733	4	1	3	4	8	217	28,777
105	209,493	158	1	1	0	0	1	158	209,493
106	179,026	857	2	0	0	9	9	95	19,892
107	191,896	1,271	5	3	1	4	8	159	23,987
108	172,930	455	2	0	0	3	3	152	57,643
109	232,811	1,142	6	3	1	5	9	127	25,868
110	174,613	802	4	0	1	5	6	134	29,102
111	183,422	2,657	5	0	0	20	20	133	9,171
112	205,437	810	2	1	0	3	4	203	51,359
113	163,692	624	4	0	1	5	6	104	27,282
114	173,085	754	3	0	2	5	7	108	24,726
115	158,618	1,640	4	1	3	5	9	182	17,624
116	160,911	427	1	1	1	0	2	214	80,456
117	159,147	691	3	4	0	1	5	138	31,829
118	164,903	2,902	7	4	5	7	16	181	10,306
119	180,073	674	3	0	0	5	5	135	36,015
120	132,002	219	1	0	0	1	1	219	132,002
121	136,681	318	2	0	0	4	4	80	34,170
122	141,975	1,011	5	1	0	8	9	112	15,775
123	142,526	697	3	1	0	5	6	116	23,754
124	122,507	109	2	0	0	2	2	55	61,254
125	141,384	74	1	1	0	0	1	74	141,384
126	132,883	396	4	1	0	5	6	66	22,147
127	158,529	334	2	0	0	4	4	84	39,632
128	133,434	518	2	1	1	1	3	173	44,478
129	156,670	102	1	0	0	1	1	102	156,670
130	127,762	653	2	0	0	3	3	218	42,587
131	114,788	871	3	1	1	5	7	124	16,398
132	135,433	436	4	2	0	2	4	109	33,858
133	128,998	576	3	0	1	3	4	144	32,250
135	163,446	463	2	1	0	2	3	154	54,482
137	105,823	42	1	0	0	1	1	42	105,823
138	90,221	617	2	2	2	0	4	154	22,555
139	93,586	451	3	0	1	6	7	64	13,369
140	96,224	620	1	1	0	0	1	620	96,224
141	98,031	1,362	4	0	0	14	14	97	7,002
142	132,305	323	2	0	0	4	4	81	33,076
143	87,623	181	1	0	0	1	1	181	87,623
144	85,648	237	1	0	0	2	2	119	42,824
145	74,269	118	2	0	0	2	2	59	37,135
146	66,839	291	1	1	0	0	1	291	66,839
147	66,397	266	2	1	0	2	3	89	22,132
148	64,884	982	3	3	4	2	9	109	7,209
150	63,036	779	3	4	2	0	6	130	10,506
151	78,814	2,274	4	0	0	12	12	190	6,568
152	68,096	1,607	2	0	0	11	11	146	6,191
153	58,505	159	1	0	0	1	1	159	58,505
154	59,544	409	0	2	0	1	3	136	19,848
155	57,757	158	1	0	0	1	1	158	57,757
156	63,803	440	1	0	0	2	2	220	31,902
159	48,833	198	2	0	0	2	2	99	24,417
161	70,147	322	1	0	0	3	3	107	23,382
162	96,527	127	2	1	0	1	2	64	48,264
163	42,923	1,510	1	1	0	5	6	252	7,154
164	56,756	571	1	0	0	5	5	114	11,351
165	46,219	239	1	0	0	5	5	48	9,244
166	62,331	189	1	0	0	1	1	189	62,331
167	55,078	172	1	0	0	3	3	57	18,359
169	56,434	309	1	0	0	3	3	103	18,811
170	41,057	492	2	0	0	4	4	123	10,264
171	47,090	263	1	0	0	2	2	132	23,545
172	51,493	74	1	0	0	1	1	74	51,493
173	32,945	87	1	0	0	2	2	44	16,473
178	50,831	239	1	1	1	0	2	120	25,416
180	26,426	118	1	0	0	1	1	118	26,426
181	25,288	543	1	0	1	5	6	91	4,215
183	25,255	54	1	0	0	1	1	54	25,255
184	25,182	550	1	2	0	1	3	183	8,394
189	22,821	40	1	1	0	0	1	40	22,821
191	21,049	312	1	0	0	1	1	312	21,049
193	20,340	312	1	0	0	1	1	312	20,340
195	20,131	38	1	0	0	1	1	38	20,131
197	19,986	343	1	0	1	0	1	343	19,986
202	18,478	80	1	0	0	1	1	80	18,478
203	18,097	65	1	1	0	0	1	65	18,097
204	19,139	43	1	0	0	1	1	43	19,139
205	19,822	671	2	0	0	6	6	112	3,304
207	17,451	498	1	0	0	4	4	125	4,363
209	16,612	166	1	0	0	1	1	166	16,612
210	16,297	163	1	0	0	1	1	163	16,297
213	15,902	189	1	0	0	2	2	95	7,951
214	16,066	603	1	0	0	3	3	201	5,355
216	15,718	318	1	0	0	2	2	159	7,859
219	15,090	55	1	0	0	1	1	55	15,090
220	14,899	34	1	0	1	0	1	34	14,899
221	14,629	71	1	0	0	2	2	36	7,315
225	13,856	702	1	2	1	0	3	234	4,619
227	13,483	124	1	1	0	0	1	124	13,483
228	13,460	488	1	0	0	3	3	163	4,487
236	12,526	158	1	0	0	1	1	158	12,526
237	12,336	1,760	1	0	0	8	8	220	1,542
238	12,284	618	1	2	0	0	2	309	6,142
239	15,052	80	1	1	0	0	1	80	15,052
241	12,067	1,285	1	3	1	4	8	161	1,508
246	11,389	538	1	0	1	0	1	538	11,389
249	10,855	35	1	1	0	0	1	35	10,855
256	10,451	546	1	0	0	3	3	182	3,484
262	10,127	78	1	0	0	1	1	78	10,127
263	10,113	277	1	0	0	5	5	55	2,023
265	10,029	532	1	0	0	4	4	133	2,507
267	9,743	129	1	0	0	1	1	129	9,743
268	9,738	215	1	0	1	2	3	72	3,246
270	9,523	125	1	0	0	1	1	125	9,523
271	9,385	101	1	0	0	2	2	51	4,693
273	9,255	96	1	0	0	1	1	96	9,255
276	8,976	139	1	0	1	0	1	139	8,976
278	8,937	70	1	0	0	1	1	70	8,937
286	8,437	50	1	0	0	1	1	50	8,437
287	8,404	215	1	0	0	3	3	72	2,801
291	8,194	79	1	0	0	1	1	79	8,194
292	8,128	469	1	0	0	4	4	117	2,032
293	8,101	545	1	0	0	5	5	109	1,620
295	8,046	863	1	1	1	0	2	432	4,023
298	7,881	91	1	0	0	1	1	91	7,881
299	7,678	2,047	1	0	0	5	5	409	1,536
307	7,428	53	1	0	0	1	1	53	7,428
311	7,373	83	1	0	0	1	1	83	7,373
313	7,328	154	1	1	0	0	1	154	7,328
325	6,734	338	1	0	0	2	2	169	3,367
331	6,471	370	1	3	1	0	4	93	1,618
340	6,192	281	1	0	0	3	3	94	2,064
351	5,919	167	1	1	0	0	1	167	5,919
359	5,526	604	1	0	0	3	3	201	1,842
360	5,501	281	1	0	0	1	1	281	5,501
363	5,438	477	1	0	0	4	4	119	1,360
368	5,333	375	1	0	0	2	2	188	2,667
371	5,291	383	1	0	0	4	4	96	1,323
374	5,126	432	1	0	0	3	3	144	1,709
375	5,108	266	1	0	0	1	1	266	5,108
376	5,100	304	1	0	0	5	5	61	1,020
377	5,096	63	1	0	1	0	1	63	5,096
384	4,789	30	1	0	0	1	1	30	4,789
386	4,740	98	1	0	0	1	1	98	4,740
392	2,878	39,740	1	76	12	62	150	265	19
Total	86,550,604	787,413	1,447	1,088	572	2,952	4,604	35,082	18,799