Molecular Characterization and Tissue Localization of an F-Box Only Protein from Silkworm, Bombyx mori

Figure 2

Purification of protein fractions from E. coli BL21(DE3) expressing recombinant BmFBXO21. Samples were resolved by12% SDS-PAGE under reducing conditions (lane 1: lysate from E. coli without induction, lane 2: lysate from E. coli after induction, lane 3: lysate from recombinant E. coli after induction, lane 4: supernatant from supersonic fragmentation, lane 5: deposition of supersonic fragmentation, lane 6: purified recombinant BmFBXO21, lane M: size markers).