Anaplasma phagocytophilum and Anaplasma marginale Elicit Different Gene Expression Responses in Cultured Tick Cells
Figure 2
Effect of A. phagocytophilum and A. marginale infection on tick ISE6 cells gene expression. Total RNA was extracted from three A. marginale-infected, three A. phagocytophilum-infected, and three uninfected ISE6 cell cultures. The expression fold change was determined by microarray hybridization at 6 days postinfection (dpi) (approximately 70% infected cells; companion cultures were terminal at 8 dpi). Uninfected cells were sampled at the same time point as infected cells to account for culture time effects. Ratios were calculated as Anaplasma-infected cells versus uninfected control cells. Normalized ratio values obtained for each probe were averaged across 3 biological replicates and four technical replicates and only entries displaying a significant () expression fold change >2 in either A. phagocytophilum- or A. marginale-infected cells are shown. Clone ID (library plate and well) are shown. The graph was constructed with the HCE software (http://www.cs.umd.edu/hcil/hce/hce3.html).
