Characterization of the Gene BmEm4, a Homologue of Drosophila E(spl)m4, from the Silkworm, Bombyx mori

Figure 7

Subcellular localization of BmEm4 with Cy3-labeled goat antirabbit IgG and DAPI. (a)–(d), (e)–(h), (i)–(l) correspond to three individual experimental groups, which were stained with the anti-BmEm4 polyclonal antibody. (a) and (e) the subcellular localization of BmEm4 as indicated by the Cy3-labeled secondary antibody; (b) and (f) DAPI staining; (c) and (g) show the previous two images merged together; (i)–(l) negative control group, using control rabbit serum; (l), signal obtained with Cy3-labeled secondary antibody; (j) DAPI staining; (k), merged images from (i) and (j); (d), (h), (l) are bright field images.

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