Research Article
Computational Identification of Novel MicroRNAs and Their Targets in Vigna unguiculata
Table 1
New miRNAs identified in Vigna unguiculata.
| | New miRNA | Gene ID | Source | miRNA sequence () | NM/nt | LM/nt | LP/nt | Location | A + U (%) | MFEI |
| | vun-MIR156a | 146512093 | GSS | UGACAGAAGAUAGAGAGCAC | 0 | 20 | 100 | 5 | 0.58 | 1.06 | | vun-MIR156b | 146512093 | GSS | CAGAAGAUAGAGAGCACAGA | 2 | 20 | 92 | 5 | 0.58 | 1.06 | | vun-MIR156c | 146512093 | GSS | UUGACAGAAGAUAGAGAGCAC | 0 | 21 | 100 | 5 | 0.59 | 1.06 | | vun-MIR156d | 146512093 | GSS | UGACAGAAGAUAGAGAGCACA | 1 | 21 | 100 | 5 | 0.58 | 1.06 | | vun-MIR156e | 190469749 | EST | CAGAAGAUAGAGAGCACAGA | 2 | 20 | 77 | 5 | 0.58 | 1.18 | | vun-MIR156f | 190510876 | EST | UGACAGAAGAGAGUGAGCACA | 0 | 21 | 82 | 5 | .055 | 1.09 | | vun-MIR156g | 190510876 | EST | UUGACAGAAGAGAGUGAGCAC | 3 | 21 | 84 | 5 | 0.55 | 1.07 | | vun-MIR156h | 190510876 | EST | UGACAGAAGAGAGUGAGCAC | 0 | 20 | 82 | 5 | 0.55 | 1.09 | | vun-MIR156i | 190416464 | EST | UGACAGAAGAGAGUGAGCACA | 0 | 21 | 82 | 5 | 0.57 | 1.29 | | vun-MIR156j | 190416464 | EST | UUGACAGAAGAGAGUGAGCAC | 3 | 21 | 84 | 5 | 0.58 | 1.30 | | vun-MIR156k | 190416464 | EST | UGACAGAAGAGAGUGAGCAC | 0 | 20 | 82 | 5 | 0.57 | 1.29 | | vun-MIR157a | 190469749 | EST | UUGACAGAAGAUAGAGAGCAC | 1 | 21 | 84 | 5 | 0.60 | 1.32 | | vun-MIR157b | 190469749 | EST | UGACAGAAGAUAGAGAGCAC | 0 | 20 | 84 | 5 | 0.60 | 1.31 | | vun-MIR157c | 190469749 | EST | UGACAGAAGAUAGAGAGCACA | 1 | 21 | 84 | 5 | 0.60 | 1.31 | | vun-MIR159 | 182406416 | EST | CUUGGACUGAAGGGAGCUCCU | 1 | 21 | 186 | 3 | 0.60 | 1.00 | | vun-MIR160a | 190509770 | EST | UGCCUGGCUCCCUGUAUGCCA | 0 | 21 | 81 | 5 | 0.48 | 1.05 | | vun-MIR160b | 190509770 | EST | UGCCUGGCUCCCUGUAUGCC | 0 | 20 | 81 | 5 | 0.48 | 1.05 | | vun-MIR164 | 146508076 | GSS | UGGAGAAGCAGGGCACGUGCA | 0 | 21 | 68 | 5 | 0.41 | 0.90 | | vun-MIR169a | 190499818 | EST | CAGCCAAGGAUGACUUGCCGGC | 2 | 22 | 76 | 5 | 0.46 | 1.04 | | vun-MIR169b | 190499818 | EST | CAGCCAAGGAUGACUUGCCGG | 0 | 21 | 76 | 5 | 0.46 | 1.04 | | vun-MIR169c | 190499818 | EST | GCAGCCAAGGAUGACUUGCCG | 1 | 21 | 77 | 5 | 0.46 | 1.02 | | vun-MIR172a | 190415307 | EST | AGAAUCUUGAUGAUGCUGCAUCAG | 2 | 24 | 109 | 3 | 0.55 | 1.02 | | vun-MIR172b | 190415307 | EST | UGAAUCUUGAUGAUGCUACAU | 0 | 21 | 101 | 3 | 0. 57 | 0.89 | | vun-MIR172c | 190415307 | EST | AGAAUCUUGAUGAUGCUGCAU | 0 | 21 | 101 | 3 | 0.57 | 1.03 | | vun-MIR172d | 190415307 | EST | UGAAUCUUGAUGAUGCUACA | 2 | 20 | 100 | 3 | 0.57 | 0.87 | | vun-MIR172e | 190415307 | EST | AGAAUCUUGAUGAUGCUGCA | 1 | 20 | 101 | 3 | 0.56 | 0.99 | | vun-MIR319a | 182650666 | EST | UUGGACUGAAGGGAGCUCCCU | 0 | 21 | 172 | 3 | 0.58 | 0.99 | | vun-MIR319b | 182650666 | EST | CUUGGACUGAAGGGAGCUCCC | 0 | 21 | 174 | 3 | 0.59 | 1.03 | | vun-MIR319c | 182650666 | EST | UUGGACUGAAGGGAGCUCCC | 0 | 20 | 174 | 3 | 0.59 | 1.03 | | vun-MIR319d | 182650666 | EST | CUUGGACUGAAGGGAGCUCC | 0 | 20 | 167 | 3 | 0.58 | 1.01 | | vun-MIR319e | 182650666 | EST | UUGGACUGAAGGGAGCUCC | 0 | 19 | 167 | 3 | 0.58 | 1.01 | | vun-MIR319f | 182406416 | EST | UUGGACUGAAGGGAGCUCCUU | 0 | 21 | 188 | 3 | 0.59 | 1.00 | | vun-MIR319g | 182406416 | EST | CUUGGACUGAAGGGAGCUCC | 0 | 20 | 184 | 3 | 0.58 | 1.00 | | vun-MIR319h | 182406416 | EST | UUGGACUGAAGGGAGCUCCU | 0 | 20 | 186 | 3 | 0.59 | 1.00 | | vun-MIR319i | 182406416 | EST | UUGGACUGAAGGGAGCUCC | 0 | 19 | 184 | 3 | 0.58 | 0.98 | | vun-MIR395a | 146525281 | GSS | CUGAAGUGUUUGGGGGAACUCC | 0 | 22 | 81 | 3 | 0.59 | 1.04 | | vun-MIR395b | 146525281 | GSS | CUGAAGUGUUUGGGGGAACUC | 0 | 21 | 80 | 3 | 0.60 | 1.04 | | vun-MIR399a | 182643512 | EST | UGCCAAAGGAGAAUUGCCCUG | 0 | 21 | 77 | 3 | 0.52 | 0.91 | | vun-MIR399b | 182643512 | EST | UGCCAAAGGAGAAUUGCCC | 0 | 19 | 77 | 3 | 0.52 | 0.91 | | vun-MIR399c | 182648023 | EST | UGCCAAAGGAGAGUUGCCCUG | 0 | 21 | 73 | 3 | 0.56 | 1.16 | | vun-MIR399d | 182648023 | EST | UGCCAAAGGAGAGUUGCCC | 1 | 19 | 69 | 3 | 0.55 | 1.11 | | vun-MIR482 | 146504713 | GSS | UCUUCCCAAUUCCGCCCAUUCCUA | 0 | 24 | 80 | 3 | 0.56 | 0.96 | | vun-MIR1507a | 182400468 | EST | UCUCAUUCCAUACAUCGUCUGA | 0 | 22 | 88 | 3 | 0.53 | 1.03 | | vun-MIR1507b | 182400468 | EST | UCUCAUUCCAUACAUCGUCUG | 0 | 21 | 87 | 3 | 0.53 | 1.03 | | vun-MIR1507c | 190551234 | EST | UCUCAUUCCAUACAUCGUCUGA | 0 | 22 | 88 | 3 | 0.55 | 1.18 | | vun-MIR1507d | 190551234 | EST | UCUCAUUCCAUACAUCGUCUG | 0 | 21 | 87 | 3 | 0.54 | 1.16 | | vun-MIR2118 | 190540631 | EST | UUGCCGAUUCCACCCAUUCCUA | 1 | 22 | 75 | 3 | 0.57 | 1.11 |
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NM: Number of mismatch; LM: Length of mature miRNA; LP: Length of precursor; MFEI: Minimal folding free energy index.
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