Codon Usage Patterns in Corynebacterium glutamicum: Mutational Bias, Natural Selection and Amino Acid Conservation
Table 2
Codon usage in putative highly expressed and lowly expressed genes of C. glutamicum.
AA
Codon
High
Low
AA
Codon
High
Low
RSCU
RSCU
RSCU
RSCU
Phe
UUU
149
0.17
1058
1.37
Ser
UCU
371
0.77
539
1.22
UUC
*
1608
1.83
481
0.63
UCC
*
1972
4.11
284
0.64
Leu
UUA
19
0.03
486
0.78
UCA
175
0.36
360
0.82
UUG
239
0.33
1284
2.06
UCG
59
0.12
676
1.53
CUU
656
0.91
707
1.13
Pro
CCU
527
0.92
492
1.11
CUC
*
1583
2.21
341
0.55
CCC
185
0.32
205
0.46
CUA
140
0.2
274
0.44
CCA
*
1454
2.55
371
0.84
CUG
*
1666
2.32
651
1.04
CCG
119
0.21
705
1.59
Ile
AUU
412
0.43
1207
1.71
Thr
ACU
304
0.38
605
1.2
AUC
*
2477
2.57
654
0.93
ACC
*
2758
3.45
427
0.85
AUA
7
0.01
257
0.36
ACA
74
0.09
378
0.75
Met
AUG
1143
1
863
1
ACG
61
0.08
601
1.2
Val
GUU
*
1495
1.45
883
1.11
Ala
GCU
*
1707
1.18
957
1.08
GUC
*
1631
1.59
452
0.57
GCC
*
1138
0.79
512
0.58
GUA
336
0.33
396
0.5
GCA
*
2529
1.75
789
0.89
GUG
650
0.63
1443
1.82
GCG
411
0.28
1300
1.46
Tyr
UAU
47
0.07
632
1.41
Cys
UGU
64
0.44
213
1.3
UAC
*
1311
1.93
262
0.59
UGC
*
230
1.56
114
0.7
TER
UAA
100
2.19
39
0.85
TER
UGA
4
0.09
47
1.03
UAG
33
0.72
51
1.12
Trp
UGG
577
1
661
1
His
CAU
47
0.09
560
1.35
Arg
CGU
744
1.62
557
1.45
CAC
*
981
1.91
271
0.65
CGC
*
1887
4.11
362
0.94
Gln
CAA
284
0.34
539
0.79
CGA
89
0.19
317
0.83
CAG
*
1385
1.66
830
1.21
CGG
16
0.03
489
1.27
Asn
AAU
141
0.13
795
1.37
Ser
AGU
19
0.04
483
1.09
AAC
*
1955
1.87
369
0.63
AGC
284
0.59
305
0.69
Lys
AAA
298
0.26
664
0.91
Arg
AGA
5
0.01
236
0.62
AAG
*
2014
1.74
800
1.09
AGG
13
0.03
341
0.89
Asp
GAU
959
0.55
1537
1.53
Gly
GGU
1026
0.93
1069
1.46
GAC
*
2559
1.45
468
0.47
GGC
*
2830
2.55
562
0.77
Glu
GAA
*
2117
1.04
1095
0.91
GGA
549
0.5
568
0.78
GAG
1941
0.96
1313
1.09
GGG
26
0.02
724
0.99
: the number of codons; AA: amino acid.
*Codon with significantly () higher frequencies in highly expressed genes. High: codons in highly expressed genes; Low: codons in lowly expressed genes.
