Preliminary Characterization of the Transcriptional Response of the Porcine Intestinal Cell Line IPEC-J2 to Enterotoxigenic Escherichia coli, Escherichia coli, and E. coli Lipopolysaccharide

Table 2

Primer sequences used in this study.

Symbol	Name	Probe set ID	Forward primer	Reverse primer
RPL4	Ribosomal protein L4	Ssc.12277.1.S1_at	GAGAAACCGTCGCCGAAT	GCCCACCAGGAGCAAGTT
YWHAZ	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide	Ssc.9681.1.A1_at	AGCAGATGGCTCGAGAAT	GCAACCTCAGCCAAGTAAC
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	Ssc.14942.1.S1_at	GGTCGGAGTGAACGGATTTG	ACTGTGCCGTGGAATTTGC
ACTB	Beta actin	Ssc.13874.1.S1_at	CTACGTCGCCCTGGACTTC	GATGCCGCAGGATTCCAT
B2M	Beta-2-microglobulin	Ssc.12348.1.S1_at	TCGGGCTGCTCTCACTGT	GACTGCTCCGCGTTCATC
IL8	Interleukin-8	Ssc.658.1.S1_at	TCACAAGCTCCTAGGACCAGA	CAGAACTGCAGCCTCACAGA
PAP	Pancreatitis-associated protein	Ssc.16470.1.S1_at	GAAGATTCCCCAGCAGACAC	AGGACACGAAGGATGCCTC
FABP1	Liver fatty acid binding protein	Ssc.604.1.S1_at	CCAAGTACAGAGCCAGGAAAA	CCCGGTAGTGATGGTCAACT
FABP2	Intestinal fatty acid binding protein	Ssc.16525.1.S1_at	TGAATCAGCTGGAGACTATGG	TTTACCACGTTAATACCCATTTTT
FABP5	Epidermal fatty acid binding protein	Ssc.5549.1.S1_at	CCAGGCTCTAGGCACCAGT	GGCCATTCCCACTCCTACTT
IRG6	Inflammatory response protein 6	Ssc.286.1.S1_s_at	CATCAATCGCTTCAATGTGG	ACCAAGCAGGACACGTCTTT
CYP1A1	Cytochrome P450 1A1	Ssc.208.1.S1_at	CAACACGTCCCTGGATCTCT	ATCCGACAGCTGGATATTGG