Preliminary Characterization of the Transcriptional Response of the Porcine Intestinal Cell Line IPEC-J2 to Enterotoxigenic Escherichia coli, Escherichia coli, and E. coli Lipopolysaccharide

Table 4

Microarray data expressed as a log2 fold change of the transcriptional response of IPEC-J2 cocultured with inflammatory stimuli as compared to the control. Genes common to at least two out of four treatments are given (for full list see Supplementary Material).

	log2 fold change
Probeset ID	LPS versus control	CVI-444 versus control	CVI-1000 versus control	CVI-1048 versus control	Public ID	Gene title	Gene symbol	Tentative function (UniProtKB)
Ssc.19163.1.S1_at	−𝟏,𝟓𝟏	−𝟏,𝟒𝟐	−𝟏,𝟏𝟎	−𝟏,𝟕𝟏	BF078930	Transcribed locus	—
Ssc.19268.2.A1_at	−𝟏,𝟎𝟗	−𝟏,𝟎𝟗	−𝟏,𝟏𝟏	−𝟏,𝟏𝟔	CF359637	Transcribed locus, moderately similar to NP_660274.1 chromosome 14 open reading frame 143 (H. sapiens)	—	Calcium ion binding
AFFX-SSC-28SrRNA_at	−𝟏,𝟏𝟔	−𝟏,𝟒𝟔	−𝟏,𝟐𝟔	−𝟏,𝟐𝟎	AFFX-SSC-28SrRNA	S. scrofa 28S rRNA	—
Ssc.24007.1.S1_at	𝟐,𝟏𝟔	𝟏,𝟎𝟒	𝟏,𝟑𝟖	𝟏,𝟔𝟖	CK450048	Paternally expressed 10	PEG10	Nucleic acid binding
Ssc.15937.1.A1_at	𝟏,𝟓𝟎	𝟏,𝟓𝟒	𝟏,𝟒𝟏	𝟏,𝟔𝟐	AF248308.1	Transcribed locus, moderately similar to XP_375341.1 similar to Ig heavy chain - human (fragment) (H. sapiens)	—
Ssc.19692.1.S1_at	𝟏,𝟑𝟒		𝟏,𝟖𝟕	𝟏,𝟗𝟐	BF078671	Chemokine (C-X-C motif) ligand 2	CXCL2	Immune response
Ssc.4871.1.S1_at	𝟏,𝟖𝟓		𝟐,𝟐𝟏	𝟐,𝟓𝟕	NM_001001861.1	Chemokine (C-X-C motif) ligand 2	CXCL2	Immune response
Ssc.658.1.S1_at	𝟏,𝟗𝟑		𝟐,𝟐𝟗	𝟐,𝟏𝟖	NM_213867.1	Interleukin 8	IL8	Cytokine, inflammatory response, immune response
Ssc.14467.2.S1_a_at	𝟏,𝟗𝟗	𝟏,𝟐𝟒	𝟏,𝟏𝟒		AY028311.1	Amphiregulin	AREG	Cytokine, growth factor
Ssc.286.1.S1_s_at	−𝟏,𝟕𝟐			−𝟏,𝟖𝟒	NM_213817.1	Inflammatory response protein 6	IRG6	Antiviral defense, defense response to virus
Ssc.208.1.S1_at	𝟐,𝟐𝟐	𝟐,𝟕𝟓	𝟏,𝟖𝟑		NM_214412.1	Cytochrome P450 1A1	CYP1A1	Oxidation reduction
Ssc.113.1.S2_at			𝟏,𝟏𝟐	𝟐,𝟏𝟔	NM_214029.1	Interleukin 1, alpha	IL1A	Cytokine, inflammatory response, immune response
Ssc.113.1.S1_at			𝟏,𝟐𝟖	𝟐,𝟓𝟖	M86730.1	Interleukin 1, alpha	IL1A	Cytokine, inflammatory response, immune response
Ssc.10453.1.S1_at			−𝟏,𝟎𝟕	−𝟏,𝟒𝟐	BF713714	Transcribed locus, highly similar to NP_000087.1 ceruloplasmin (ferroxidase) (H. sapiens)	—	Oxidoreductase, transport
Ssc.26317.1.S1_at	𝟏,𝟐𝟑			−𝟏,𝟓𝟓	AY509877.1	Alpha-2-macroglobulin	A2M	Endopeptidase inhibitor activity
Ssc.7090.1.A1_at	𝟏,𝟒𝟔			−𝟏,𝟒𝟖	NM_214395.1	Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1	SERPINA1	Protease inhibitor, serine protease inhibitor
Ssc.16053.1.S1_at				−𝟏,𝟎𝟖	AF069643.1	Matrix metalloproteinase 13 precursor	MMP-13	Hydrolase, protease, metalloprotease
Ssc.4368.3.S1_at	−𝟏,𝟕𝟑			−𝟏,𝟏𝟗	BP463181	F-box protein 32	FBXO32	Ubl conjugation pathway
Ssc.4368.1.S1_at	−𝟏,𝟓𝟗			−𝟏,𝟑𝟐	BI817204	F-box protein 32	FBXO32	Ubl conjugation pathway
Ssc.221.1.S1_at	−𝟏,𝟎𝟗			−𝟏,𝟓𝟓	NM_214061.1	Myxovirus (influenza virus) resistance 1, interferon-inducible protein p78	MX1	Antiviral defense, reponse to virus
Ssc.21.1.S1_s_at	−𝟏,𝟎𝟓			−𝟏,𝟔𝟔	AF319661.1	DEAD (Asp-Glu-Ala-Asp) box polypeptide 58	DDX58	Antiviral defense, immune response, innate immunity
Ssc.15888.1.S1_at	−𝟏,𝟐𝟐			−𝟏,𝟎𝟕	NM_213805.1	Oxidized low density lipoprotein (lectin-like) receptor 1	OLR1	Cell adhesion, immune response, inflammatory response
Ssc.16648.1.S1_at	−𝟏,𝟐𝟖	−𝟏,𝟑𝟓	−𝟐,𝟑𝟎	−𝟑,𝟑𝟒	CK458095	Thioredoxin interacting protein	TXNIP	Cell cycle, transcription, transcription regulation