Research Article
mRNA/microRNA Profile at the Metamorphic Stage of Olive Flounder (Paralichthys olivaceus)
Table 1
Primers of mRNAs for qRT-PCR.
| | Clone name | Gene name | Primer name | Primer (5′-3′) |
| | SFU-PO-DEV 0004 | 5-cytosolic ii (nt5c2) | CY2F | CGACCTACCTGCCAACATG | | CY2R | GTGCCAGACAACTGGTCC | | SFU-PO-DEV 0125 | cog1782: metal-dependent consists of a metallo-beta-lactamase domain and an rna-binding kh domain (me) | MEF | CTGACCAAACTGATCCGGC | | MER | CTGATCCAACATCGAGGTCG | | SFU-PO-DEV 0133 | Creatine kinase 1 mRNA (ck1) | CKF | GAGACTCGTGACTCTGCTCAC | | CKR | CAGCCTAGTGGAGGCTGATC | | SFU-PO-DEV 0137 | Cuticle protein (cp) | CP2F | GTGGCTACAACGCCGATG | | CPR | CAGGTGCTTTGTATGCAGGAG | | SFU-PO-DEV 0159 | Cytochrome c subunit va (cox5a) | CSF | GCAGCACTGAGAGCTTGTC | | CSR | GCTGCAGCTCTTGGATCAG | | SFU-PO-DEV 0170 | Deoxycytidine kinase (dck) | DK2F | GACTCCAAGCCCGGAACC | | DK2R | AGCTGAAGGCACAGCTAGTG | | SFU-PO-DEV 0182 | Elastase 2a (ela2a) | EL2F | GACCTGGCCAGTTGCAATG | | EL2R | GTAGTTGCAGCCCATGCTTG | | SFU-PO-DEV 0200 | Eukaryotic translation initiation factor 5A-2 (eif5a2) | ETF | CCATCGGCATCAAAGCCTTG | | ETR | GAGTAGCATTGACGAGGCAATG | | SFU-PO-DEV 0213 | Fatty acid binding protein intestinal (fabpi) | FAPF | CTATCAGGCTCGTGGACCATG | | FAPR | CTTTGCGTCCACACCTTCG | | SFU-PO-DEV 0260 | Heat shock protein 70 (hsp71) | HSPF | GCTACCTCCTGAGAAAGTGCTC | | HSPR | GTGACTCACTGCTCACTGAGTC | | SFU-PO-DEV 0283 | Danio rerio hypothetical LOC562892 (hy) | HYF | GCTGAGCTGCACTGATCAAG | | HYR | TGCTGCATGTGCACACTTG | | SFU-PO-DEV 0295 | Kiaa1872 protein (atcay) | KP2F | CGTCTTCCAGGCGACAG | | KP2R | GTACGGCCTGATGACCTG | | SFU-PO-DEV 0297 | l-3-hydroxyacyl-coenzyme ashort chain (hadh) | HCF | CAGAGTGAGCTGCTGAACAAG | | HCR | TGAAGTTCGGACTTGTCCCTC | | SFU-PO-DEV 0298 | Larval and non-ifm isoform (myl) | LIF | GCATGGATCCTGAAGATGACG | | LI2R | CAGGGTAAGGTCCAGCAATG | | SFU-PO-DEV 0305 | Lin-52 homolog (lin52) | LHF | GTTCTTCAAGTGGCGGGAC | | LHR | GCTGCAGTTCACTGTCTGAG | | SFU-PO-DEV 0341 | Middle subunit (ferritin m) (hfe) | FMF | GATTGAGCCTGAAGGGACGAG | | FMR | CGACACCAACACAACAGCTC | | SFU-PO-DEV 0766 | Nadh dehydrogenase subunit 4l (mt-nd4l) | NDF | CTCAGCCAACTTCTCAGCTTC | | NDR | GCTTTGAAGTCGGTCGGTAC | | SFU-PO-DEV 0788 | Nonmetastatic cells protein expressed in isoform a (nme1) | NMC-2F | GACTCCAAGCCCGGAACA | | NMC-2R | CAGTGGTCTCCGACCAGG | | SFU-PO-DEV 0803 | Ornithine decarboxylase antizyme small isoform (oaz) | ODAF | CGGGATCGCAATCTTTCAGC | | ODAR | GAGCAAGAAGCGCACTCTG | | SFU-PO-DEV 0834 | Putative cytochrome P450 like protein precursor (cyp1a1) | PCPF | GTCAAGCTCAACAGGCTCTTC | | PCPR | TGACGCGCATGAATGGATG | | SFU-PO-DEV 0914 | Ribosomal protein s27 (rps27) | RPF | CGGTTGATATCAGCGCCTTG | | RPR | CGATCTGTCAACGCGAACAG | | SFU-PO-DEV 0945 | s-adenosylhomocysteine hydrolase (ahcyl) | SAHF | GTACCTGGGTCTGCCCAG | | SAHR | CCAAACGCCAAACCCTTCTAG | | SFU-PO-DEV 1002 | Translocase of inner mitochondrial membrane 8 homolog a (timm8a) | TIMMF | TCCGAGGGCATGATGGAC | | TIMMR | GGACAGTTCTGCAACACTCAG | | SFU-PO-DEV 1017 | Troponin c type 2 (tnnc2) | TNNCF | CTACTGACCCCACTGTACCAC | | TNNCR | CCGTCTGTTGAGGATGTCAATG |
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