Repertoire of SSRs in the Castor Bean Genome and Their Utilization in Genetic Diversity Analysis in Jatropha curcas

Table 2

SSRs used to detect polymorphisms in Jatropha species.

SSR locus	Repeat motif	Primer sequence	Location of repeat motif	No. of alleles detected	HO	HE	PIC
JM8	(TC)23	F: GAATTTAGAAGCCACATTTGAGACG; R: CCTATGTAACCCAAGAAAGACGATG	Intron	3	0.7654	0.7654	0.7
JM10	(GAA)15	F: TGGAAGACGAATACTATGACGATGA; R: CAGGTGCTACTTCTTCTTCTTCAGG	Exon	2	0.5925	0.5926	0.5
JM11	(CAT)12	F: GCATGCAAACCCTGAATTATGTACT; R: GCTGCTGACTCTGTTTCTCCTTCTA	Exon	2	0.5679	0.5679	0.5
JM15	(TA)42	F: TGTAGATAGCCTTAGCTGTGCATTG; R: GTACTCTCGAGGGAGTTGATTGTGT	Non genic	2	0.4938	0.4938	0.4
JM16	(TA)38	F: TTGGCTCATAATAACTCCTCAAAGC; R: GCGAGTGCTGTCTAAAGCCTAATTT	Non genic	2	0.6913	0.6914	0.6
JM17	(TA)31	F: CTTCTCAGCAACATCACATCAAACT; R: CGCTAAGTTACATAGGACAAAGGGA	5′UTR	3	0.6172	0.6173	0.6
JM18	(AT)25	F: ATTCAGGCCATCCACATAGTCTAAC; R: GACCCTATTGATTGATTTAAGAGCC	5′UTR	2	0.5925	0.5926	0.5
JM19	(TA)33	F: GCCTTAGTTGTGCATTGCTCTATTT; R: ACTCAAACTTATGTCCCAATCGTCT	5′UTR	2	0.7407	0.7407	0.7
JM20	(TA)23	F: AGATTTAGAAATGGTAATAGGGCGG; R: GACCTATCCGTGTCGTGTAGATTT	5′UTR	7	0.6419	0.642	0.6
JM21	(AT)25	F: GCAAGAAATAAGGTACAACCGAAAC; R: GTGAGCAATTACCAAAGGAAACAAG	5′UTR	2	0.6913	0.6914	0.6
JM22	(AT)29	F: ATGCTATCGGAATAGATCCTTCGAG; R: TGGTAAACAAGAGTTGAGGGTTAGG	5′UTR	3	0.4444	0.4444	0.3
JM23	(AT)32	F: GAGATGGAAATGATTGGTGTTGAGT; R: CGCCTCATCCTCACATTATACACTT	5′UTR	3	0.7654	0.7654	0.7
JM24	(AT)20	F: TGATGGATTGAGAACTGAAGAGGAT; R: ACTCTAATTAGGCCCAGATTCCAAC	5′UTR	4	0.6913	0.6914	0.6
JM25	(CT)20	F: CTGACATATCTTATTGGGTGTGGAA; R: TGTAAGAGTATCATCCATTTGCCAG	5′UTR	2	0.6913	0.6914	0.6
JM26	(TC)18	F: GCCTTTAAGAGATTGATGGCAACTA; R: AAGTATTCATATGCCCTAAGCCTCC	3′UTR	2	0.7160	0.716	0.7
JM27	(TA)24	F: TTGGAGGTTACAATCAATGGCA; R: GCATGTGCCCGAATTGAATA	3′UTR	3	0.7654	0.7654	0.7
JM28	(AT)27	F: CCATTTGGTGTTAATCACATGAGTC; R: GACAATAGTGATGTTGGATTCCACT	3′UTR	2	0.7160	0.716	0.7
JM29	(AT)25	F: CTGTTTGAGGATCAGACTTTGAAGC; R:AAAGAAATAATGAGGAGGGAGGTTG	Intron	2	0.7160	0.716	0.7
JM30	(AT)25	F: GCATGGAAATTCAATTCTGTGCTAC; R: TTTGGTGATGAGGATTGTTGCT	Intron	2	0.7160	0.716	0.7
JM31	(ATA)19	F: TGCTCCCAGTAAGCATAAGAAGAAG; R: CTTGCTCGGTTACCATTACCATTAC	Intron	2	0.7160	0.716	0.7
JM32	(AT)22	F: AGCAAGAAACCATACTTCGAGTGTC; R: GAGATGCCAACCTTTGTGATTAGTT	Intron	2	0.6666	0.6667	0.6
JM33	(GA)21	F: TTCAATAACAGATTTGGCTAGGCTC; R: GACAATTGAAAGGTGCAATCCTAGT	Intron	3	0.4938	0.4938	0.4
JM34	(AT)24	F: CAGACCCATCTGATCATCATTGTAG; R: TCCTCAGGTAAATTGCTCATCTTTC	Intron	3	0.5679	0.5679	0.5
JM35	(CT)12	F: CAGCGTCCCTCTCTCTCTTCC; R: AGGAAGTTGAGGGACCAAATTGTA	Exon	2	0.6419	0.642	0.6
JM36	(AT)29	F: GAAAGCTAGAAATCAATGAACGCAC; R: TCATTTAGTACATTGACCGGAGACA	Non genic	3	0.6419	0.642	0.6
JM37	(AAT)16	F: AATCACATCAGTTGTAACGGCA; R: ATAATCTGATGGTTCAGTCAGCTCC	Non genic	3	0.7407	0.7407	0.7