Research Article

Proteomic Analysis of Larval Midgut from the Silkworm (Bombyx mori)

Table 1

List of proteins identified from midgut of Bombyx mori.

Protein classificationSpot no.Protein descriptionAccession numberMW (kDa)/ theory PICoverage
(%)
V4/V4N
(F/N)
V5/V5N
(F/N)
V6/VN
(F/N)
V7/V7N
(F/N)
V8/V8N
(F/N)
V9N
(N)
V10N
(N)
V11N
(N)
V12N
(N)
V13N
(N)

Nutrition storage830 kDa protein [Bombyx mori]ACO3575029.83/5.9023.8 + / + + / + + / + + / + + / + + + + + +
3730 K lipoprotein precursor [Bombyx mori]CAA3043330.26/7.0832.6 / + / + / + / /
38Low-molecular-mass 30 kDa lipoprotein 21G1Precursor [Bombyx mori]CAA3853132.98/6.1926.5 / / / + / + + / + + + + + +
57Low-molecular-mass 30 kDa lipoprotein 19G1 [Bombyx mori]CAA3853332.41/9.5865.5 / / / + / + / +
60Low-molecular-mass 30 kDa lipoprotein 21G1 Precursor [Bombyx mori]Q0080130.35/6.3850.2 / / / + / + + / + + + + + +
82Low molecular 30 kDa lipoprotein PBMHPC-19 precursor [Bombyx mori]NP_00109519729.41/7.2033.9 / / / + / + + + + / + + + + + +
85Low-molecular-mass 30 kDa lipoprotein 19G1 [Bombyx mori]CAA3853329.56/7.9139.1 + / / + + / + + / + + / + + + + + +
90Mature 30 K lipoprotein [Bombyx mori]CAA3043428.57/6.3744.0 / / / + / + + / + + + + +

Cell growth45Imaginal disk growth factor [Bombyx mori]BAF7362348.39/7.7928.3 / / / + + / + + + / + + + + + + + + + + + +
50Actin-depolymerizing factor1 [Bombyx mori]NP_00109327817.23/6.1941.2 + / + + / + + / + + / + / + + + + + +
75Transferrin [Bombyx mori]NP_00103701477.20/6.8427.5 / + / + + / + + / + + / + + + + + +

Protein metabolism360 kDa heat shock protein, mitochondrial [Drosophila melanogaster]NP_51111561.23/5.3723.6 + / + + / + + / + + / + + / + + + + + +
7Protein phosphatase 1 catalytic subunit [Bombyx mori]NP_00104048032.28/5.8040.5 + / + + / + + / + + / + + / + + + + + +
18Acireductone dioxygenase [Aedes aegypti]XP_001649899.21.91/4.8540.8 + / + / + / + + / + + / + + + + + +
28Glyoxylate reductase/hydroxypyruvate reductase [Culex quinquefasciatus]XP_00186566037.34/7.9432.4 / + / + / + + / + + / + + + + + +
40Protein disulfide isomerase [Aedes aegypti]ABF1837846.34/5.1133.4 / / + / + + / + + / + + + + + +
43, 44DnaJ-like protein [Bombyx mori]NP_00104018559.96/7.3229.9 / / / + / + + / + + + + + +
46Dihydrolipoamide dehydrogenase [Bombyx mori]AAM9325552.94/8.9027.8 / / / + / + + / + + + + + +
47Aspartate aminotransferase [Bombyx mori]ABF5121440.32/5.8732.3 / / / + / + / + + + + + +
52Protein disulfide-isomerase-like protein ERp57 [Bombyx mori]NP_00103699755.48/5.1737.1 / + / + + / + + + + + / + + + / + + + + + + +
58Ribosomal protein P0 [Bombyx mori]NP_00103712334.24/5.6141.8 / / / + / + + / + + + + + +
63Ubiquitin carboxyl-terminal hydrolase [Drosophila melanogaster]NP_47694025.52/4.8729.6 + / + + / + + / + + / + / + + + + + +
70Ribosomal protein P2 [Bombyx mori]NP_00103721321.20/5.7938.3 + / + + / + + / + + / + + / + + + + + +
74Heat shock protein 70 [Bombyx mori]NP_00103683773.21/4.9826.0 / + / + + / + + + / + + + / + + + + + + + + + +
87Peptidylprolyl isomerase B [Bombyx mori]NP_00104047922.39/8.9142.9 / / / + / + + / + + + + + +
9510 kDa heat shock protein, mitochondrial [Lepeophtheirus salmonis]ACO1179611.18/7.6849.5 / / / / + / + + + + +

Carbohydrate metabolism6Enolase [Bombyx mori]NP_00109183147.19/5.5541.6 + / + + / + + / + + / + + / + + + + + +
13Triosephosphate isomerase [Bombyx mori]AAU3418526.94/5.6050.8 + / + + / + + / + + / + + / + + + + + +
31Glyceraldehyde-3-phosphate dehydrogenase [Bombyx mori]NP_00103738635.42/8.5529.2 / + / + / + + / + + / + + + + + +

Lipid metabolism30Enoyl-CoA hydratase precursor 1 [Bombyx mori]ABD3610732.13/8.3345.9 / + / + / + + / + + / + + + + + +
35Fatty acid binding protein [Bombyx mori]NP_00103757415.01/5.0824.2 + / + + / + + + / + + + / + + + / + + + + + + + + +
36Apolipophorin-III precursor [Bombyx mori]AAB0285220.82/9.5343.0 + / + + / + + / + + / + / + + + + + +
48Acyl-coenzyme A dehydrogenase [Bombyx mori]NP_00103767247.53/7.1622.7 / / / + / + + / + + + + + +
54Acyl-coenzyme A dehydrogenase [Aedes aegypti]EAT4276635.81/7.1844.5 / / / + / + / + + + + + +
563-hydroxy acyl-CoA dehydrogenase [Bombyx mori]NP_00104041433.80/8.8728.4 / / / + / + + / + + + + +
65Lysophospholipase [Bombyx mori]NP_00104025525.97/6.9436.2 / / / + / + + / + + + + + +
79Acetoacetyl-CoA thiolase [Bombyx mori]NP_00109329640.00/7.5642.6 / / / + / + / + + + + + +
91Phosphatidylethanolamine binding protein isoform 2 [Bombyx mori]NP_00109326721.92/5.9447.7 / / / + / + + / + + + + +

Energy metabolism1H+-transporting ATP synthase beta subunit isoform 2 [Bombyx mori]NP_00104170554.89/5.2037.8 + / + + / + + / + + / + + / + + + + + +
5H+-transporting ATP synthase beta subunit isoform 1 [Bombyx mori]NP_00104045055.04/5.1333.9 + / + + / + + / + + / + + / + + + + + +
15Abnormal wing disc-like protein [Bombyx mori]NP_00109328417.37/7.3048.1 + + / + + + + / + + + + + / + + + + / + + + / + + + + + + + + + + + + + + +
20Vacuolar ATP synthase subunit F [Bombyx mori]NP_00104044813.92/5.9250.0 + / + / + / + + / + + / + + + + + +
22Cytochrome c oxidase subunit Va [Bombyx mori]NP_00110674217.24/5.5931.8 + / + + / + + / + + / + + / + + + + +
23H+-transporting ATP synthase delta subunit [Bombyx mori]NP_00109309117.16/5.6649.1 + / + + / + + / + + / + + / + + + + + +
33H+-transporting ATP synthase subunit d [Bombyx mori]NP_00109327920.20/5.4939.7 + / + + / + + / + / /
51Vacuolar ATPase B subunit [Bombyx mori]ACE7827155.19/5.2038.1 / / + / + + + + / + + + / + + + + + + +
53Ubiquinol-cytochrome c reductase [Bombyx mori]ABF6022529.43/8.5223.1 / / / + / + + / + + + + + +
55Vacuolar ATP synthase catalytic subunit A [Bombyx mori]NP_00109182968.60/5.1338.7 / + / + + / + + + + / + + + / + + + + + + + + + + + +
64ATP synthase subunit alpha [Bombyx mori]BAA0372528.66/8.4341.9 / / / + / + + / + + + + + +
66ATP synthase subunit alpha [Bombyx mori]BAA0372528.66/8.4341.9 / / / + + / + + + / + + + + + + + +
714-Hydroxybutyrate CoA-transferase, putative [Aedes aegypti]EAT4492143.62/6.6128.1 / / / + + + / + + + / + + + + + + + + + + + +
76Arginine kinase [Bombyx mori]ACI0104840.33/5.8436.9 + / + + / + + / + + / + + / + + + + + +
77Ubiquinol-cytochrome c reductase core protein II [Bombyx mori]NP_00110622546.18/9.5522.4 / / / + / + + / + + + + + +
86ATP synthase subunit alpha [Bombyx mori]Q0740528.66/8.4341.9 / / / + / + + / + + +
88ATP synthase subunit alpha [Bombyx mori]Q0740528.66/8.4341.9 + / + + / + + / + + / + + / + + + + + +
92NADH dehydrogenase (ubiquinone) Fe-S protein 8 [Bombyx mori]NP_00104031625.49/6.1431.2 / / / + / + + / + + + + +

Immune related4Immune-related Hdd13 [Hyphantria cunea]AAD0928128.38/6.8660.8 + / + + / + + / + + / + + / + + + + + +
16Cyclophilin A [Bombyx mori]NP_00103730118.10/7.9773.9 + / + + / + + / + + / + + / + + + + + +
17Cyclophilin [Bombyx mori]ABA5417318.12/7.9762.4 + / + + / + + / + + / /

Antioxidation34Glutathione S-transferase 10 [Bombyx mori]NP_00110846624.47/8.1126.7 / + / / / /
89Thiol peroxiredoxin [Bombyx mori]AAR1542022.08/6.1035.9 + / + + / + + / + + / + / + + + + + +

Muscle related2Actin A3 [Bombyx mori]CAA2819242.23/5.3831.9 + / + + / + + + + / + + + + + + / + + + + / + + + + + + + + + + + + +
9Actin, muscle-type A1 [Bombyx mori] G i | 1 1 3 2 1 6 | 42.27/5.1929.3 + / + + / + + / + + / + + / + + + + + +
14Actin, cytoplasmic A4 [Bombyx mori]NP_00111972730.05/5.8024.7 + / + + / + + / + + / + + / + + + + + +
21Myosin 1 light chain [Lonomia oblique]AAV9141122.77/4.3133.0 + / + + / + + / + + / + + / + + + + + +
39Actin, cytoplasmic A4 [Bombyx mori]NP_00111972730.05/5.8025.5 / / / + / / + + + + + +
59Beta-tubulin [Bombyx mori]NP_00103688725.18/4.5130.8 + / + + / + + / + + / + + / + + + + + +
  61Myosin heavy chain [Bombyx mori]ACQ7282596.12/4.9820.2 / / / + / + / + + +
  62Myosin light chain 2 [Bombyx mori]ACF5973522.04/4.5232.8 / / / + / / + + + + + +
67Muscular protein 20 [Bombyx mori]NP_00104047620.30/8.8145.7 / / / + / + / + +
68Transgelin [Bombyx mori]NP_00104037220.91/8.4085.0 / / / + + / + + / + + + + + +
93Myosin light chain 2 [Bombyx mori]NP_00109181322.04/4.5230.3 / / / + / + + / + + + + + +

Hormone metabolism24, 25Juvenile hormone diol kinase [Bombyx mori]NP_00103708020.79/4.4239.5 + / + + / + + / + + / + + / + + + + + +
83Putative farnesoic acid O-methyl transferase [Bombyx mori]NP_00109181526.83/6.7335.1 / / / + / + + / + + + + + +
94Putative farnesoic acid O-methyl transferase [Bombyx mori]NP_00109181524.26/5.0328.7 + / + + / + + / + + / + + / + + + + + +
96Diapause hormone precursor [Bombyx mori]BAA0375522.39/9.4460.9 / / / + / / + + +

Other proteins1027 kDa glycoprotein, p27K [Bombyx mori]Q8T11325.58/4.9525.1 + / + + / + + / + + / + + / + + + + + +
11Cellular retinoic acid binding protein [Bombyx mori]NP_00103736414.97/5.5356.8 + / + + / + + / + + / + + / + + + + + +
12Stardust, isoform B [Drosophila melanogaster]NP_57246332.15/5.8126.3 + / + + / + + / + + / + + / + + + + + +
19NADPH oxidase [Culex quinquefasciatus]XP_00185905930.17/8.3535.1 + + / + + + / + + + / + + / /
29Similar to CG31790-PA [Tribolium castaneum]XP_97196050.42/6.0122.0 / + / + / + + / / + + + + + +
32Serpin-2 [Bombyx mori]NP_00103702141.86/4.7221.1 / + / + + / + + / + / + + + + + +
41GE24263 [Drosophila yakuba]EDW9753047.04/5.9529.3 / / / + / + + / + + + + + +
42PREDICTED: hypothetical protein [Nasonia vitripennis].XP_00160257351.08/5.6828.5 / / + / + + / + + / + + + + + +
49AGAP011050-PA [Anopheles gambiae]EAA4551134.16/6.0939.7 / / / + / + + / + + + + + +
69GI18783 [Drosophila mojavensis]EDW0993918.98/4.7439.9 + / + + / + + / + + / + + / + + + + + +
72Mitochondrial aldehyde dehydrogenase [Bombyx mori]NP_00104047556.24/7.5529.6 / / / + / + + / + + + + + +
73Soluble guanylyl cyclase beta-1 subunit [Manduca sexta]AAC6126436.77/5.5520.1 / / / + / + / +
78Putative acetyl transferase [Bombyx mori]BAH9656142.01/7.8431.0 / / / + / + + / + + + + + +
80PREDICTED: similar to CG10122-PA [Tribolium castaneum]XP_97091843.23/5.0320.2 / / + / + + / + + / + + +
81Ef1alpha-like factor isoform 1 [Bombyx mori]NP_00104011831.48/7.8446.4 / / + / + + / + + / + + + + + +
84Voltage-dependent anion-selective channel [Drosophila melanogaster]NP_00103389930.13/7.4535.5 / / / + / + + / + + + + + +

Unannotated26Unknown [Bombyx mori]BGIBMGA00411612.80/4.4943.1 + / + + / + + / + / /
  27Unknown [Bombyx mori]BGIBMGA01336053.34/5.8033.1 / + / + / + + / + + / + + + + + +

Total96  37/3447/4052/5485/8775/848080827885

“+” and “−” indicate protein with and without expression, respectively. The number of “+” means the x-fold up expression compared with the “+”. “Vx” means fifth-instar day-x larvae, “F” means feeding, and “N” means nonfeeding.