Research Article

Comparison between Normalised and Unnormalised 454-Sequencing Libraries for Small-Scale RNA-Seq Studies

Table 3

Number of microsatellite repeats found in contigs produced from ten subsampled datasets of normalised and unnormalised zebra finch transcriptome libraries (means with standard errors estimated through jackknifing).

Repeat typeBloodSpleen
NormalisedUnnormalisedNormalisedUnnormalised

Dinucleotide17.8 (0.9)7.8 (0.9)30.4 (1.3)10.8 (0.5)
Trinucleotide60.1 (1.6)39.1 (1.4)72.6 (1.5)52.5 (1.6)
Tetranucleotide6.5 (0.3)3.7 (0.3)12.5 (0.7)5.5 (0.5)
Pentanucleotide0.1 (0.1)0.2 (0.1)3.5 (0.5)0.2 (0.1)
Hexanucleotide0 (0)0.1 (0.1)1.0 (0.1)0.2 (0.1)

Total84.5 (1.2)50.9 (2.0)120.0 (2.6)69.2 (1.5)