Research Article

PASSIOMA: Exploring Expressed Sequence Tags during Flower Development in Passiflora spp.

Figure 6

In situ expression patterns of putative P. suberosa genes involved in flower development. The hybridization signal is observed as a pink-red precipitate. (a, b, d, and e) show transversal sections though 5 mm long P. suberosa floral buds. (c) shows a longitudinal section. (a and b): the hybridization signal of PACEPS3005F02, a putative FRUITFULL ortholog, can be detected in the differentiating stigma (a) and in the ovule primordia (b, arrows). (b) is a lower section of the same floral bud shown in (a, c, and d): the expression of PACEPS3001E12, a putative SHATTERPROOF ortholog, is concentrated in the ovary and ovule primordia (arrow), but is also detected in the corona primordia. (d): detail of early stages of ovule primordia (arrows) showing PACEPS3001E12 expression. (e): the hybridization signal of PACEPS2001A09, a putative MEN8 ortholog, is restricted to the tapetal cells (arrow) in developing P. suberosa anthers. an: anther; co: corona primordia; ov: ovary; te: tepal primordium. Bars: (a, b, and c): 200 μm; (d): 80 μm; (e): 150 μm.
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