International Journal of Genomics / 2013 / Article / Fig 5

Research Article

Expression and Functional Analysis of Storage Protein 2 in the Silkworm, Bombyx mori

Figure 5

SDS-PAGE and Western blot analysis of SP2 protein expressed in BmN cells. (a) SDS-PAGE analysis of BmN cells lysates; (b) Western blot analysis of BmN cells lysates; (c) SDS-PAGE analysis of gradient purified SP2 protein; (d) Western blot analysis of purified SP2 protein; M: protein molecular weight marker; 1, 1′: total protein of BmN cells infected by wtbacmid; 2, 2′: total protein of the BmN cells infected by vBm-sp2; 3: protein that discharges out from the column; 4: eluted protein with 20 mM imidazole; 5: eluted protein with 250 mM imidazole; 5′: Western blot analysis of the purified fusion protein.