Research Article

Characterization of the OmyY1 Region on the Rainbow Trout Y Chromosome

Table 2

List of predicted nonrepetitive transcribed genes in rainbow trout physical map contig #6256 with corresponding EST’s in at least one database. EST designations in bold had the highest homology to OmyY1 linked sequence, and those sequences were used for homology and coverage analyses.

Gene name4 × 44 K salmonid oligo array designationcGRASP EST cluster contig annotationGenBank AnnotationMiller draft rainbow trout genome scaffoldMiller scaffold homology
(base pair identities)
Coverage in Miller scaffoldOmyY1 contig (Illumina
unless
noted)
OmyY1 contig homology
(base pair identities)
Coverage within OmyY1 contigPCR amplification in BAC cloneTranscribed gene in OmyY1 contig?

Oncorhynchus mykiss sdYAB626896.1n/an/an/a454 contig_33270/3273100%143K08/223F06Yes
F-Box/WD repeat containing protein 2 (FBXW2)C262R066/
C179R024
Contig53026MMSRT112A/
121C (overlap)
2319/2326100%Contig_22319/2326100%RT450D22/
RT492A21
Yes
Unknown (Some homology to laminin subunit beta-1-like)omyk-BX860091MMSRT112A/
121C (overlap)
747/778100%Contig_2747/778100%Likely
Na/K/2Cl co-transporter (nkcc1a)C029R137omyk-CR375193MMSRT112A832/843100%Contig_2832/843100%RT450D22Yes
Oocyte protease inhibitor-1 (opi-1)Contig59765MMSRT010A/
121C
919/940, 883/90697%,
94%
Contig_2883/90694%Likely in MMSRT010A scaffold
Unknownomyk-CB488087MMSRT010A/
121C
315/315, 311/315100%, 100%Contig_2311/315100%Likely in MMSRT010A scaffold
Unknown (Some homology to Sec16A)Contig22207MMSRT104C1193/1197100% Contig_281193/1197100%Yes
cAMP-responsive element-binding protein 3-like protein 4 (cr3l4) C264R086Contig51133MMSRT014G1173/118199%Contig_1211173/118199%RT15B10Yes
CREB-regulated transcription coactivator 2 (TORC2)C117R024MMSRT069D*657/72697%Contig_107698/748100%RT15B10Possibly
Zinc/iron-regulated protein (zip1) (splice A)C053R110MMSRT069D*391/41539%Contig_108, 1101058/1065100%RT15B10Yes
Zinc/iron-regulated protein (zip1) (splice B)C014R029CLUSTER_ID#5374769MMSRT069D*916/113799%Contig_1081125/1148100%RT15B10Likely
Unknownomyk-DV199273MMSRT069D*451/51874% Contig_135, 153696/700100%Yes
UnknownContig19333MMSRT069D*637/75587%Contig_119815/81694%Yes

Denotes scaffolds from Michael Miller’s draft genome that are paralogous to OmyY1 linked sequence.