Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells

Figure 3

Cross-amplification PCR identification of recombinant Bacmid-Slp and Bacmid-Lsp-t. M represents the DNA molecular weight marker. Lanes 1–4 represent cross-amplification PCR products of the recombinant Bacmid-Slp with M13F and M13R, M13F and P1′, M13R and P1, and P1 and P1′ as primers, respectively (see Section 2). Lanes 5–8 represent cross-amplification PCR products of the recombinant Bacmid-Lsp-t with M13F and M13R, M13F and P2′, M13R with P2, and P2 and P2′ as primers, respectively.