A New Exon Derived from a Mammalian Apparent LTR Retrotransposon of the SUPT16H Gene
Figure 3
RT-PCR analysis of the SUPT16H transcript in human and primate tissues. (a) The SUPT16H transcript is expressed broadly in all of the tissues assayed in humans, crab-eating monkey, marmoset, and squirrel monkey. The grey line indicates the position of the primer pair (S: forward primer; AS: reverse primer), and the expected product size is 405 bp. In this analysis, a new alternative transcript was detected that was 654 bp long. It was also expressed broadly. Tissues assayed in humans: M: marker; upper Lane 1: adrenal gland; Lane 2: cerebellum; Lane 3: adult whole brain; Lane 4: heart; Lane 5: kidney; Lane 6: liver; Lane 7: lung; Lane 8: testis; Lane 9: trachea; Lane 10: bone marrow; lower Lane 1: fetal brain; Lane 2: fetal liver; Lane 3: placenta; Lane 4: prostate; Lane 5: salivary gland; Lane 6: skeletal muscle; Lane 7: spinal cord; Lane 8: thymus; Lane 9: thyroid; and Lane 10: uterus. Tissues assayed in crab-eating monkey: Lane 1: cerebrum; Lane 2: cerebellum; Lane 3: pituitary gland; Lane 4: heart; Lane 5: lung; Lane 6: spleen; Lane 7: liver; Lane 8: pancreas; Lane 9: kidney; and Lane 10: urinary bladder. Tissues assayed in marmoset: Lane 1: heart; Lane 2: lung; Lane 3: brain; Lane 4: stomach; Lane 5: liver; Lane 6: kidney; Lane 7: pancreas; Lane 8: large intestine; Lane 9: spleen; and Lane 10: small intestine. Tissues assayed in squirrel monkey: Lane 1: heart; Lane 2: esophagus; Lane 3: interbrain; Lane 4: small intestine; Lane 5: lung; Lane 6: pancreas; Lane 7: cerebrum; Lane 8: large intestine; Lane 9: stomach; and Lane 10: interbrain. GAPDH was amplified as a positive control in all instances. (b) The alignment of the SUPT16H transcript, new alternative transcript, and EST (BX333035). The alternative transcript has a cassette exon derived from the MaLR (grey box).
