Figure 2: Real-time RT-qPCR analysis of IL-1β, TNF-α, IL-6, TGF-β (a), lysozyme, C3, factor B, and factor H (b) in the liver of O. mykiss 1 (open bars), 5 (gridded bars), and 10 (closed bars) days after ip implantation of coconut oil alone (control) or containing 50 μg/g bw cortisol. Relative expression levels were normalized to 18S rRNA and fold changes were calculated against control. Values represent the means and SEM ( per group). Significant differences are highlighted as *versus control ( ). The horizontal line originating at denotes the control group against which the expression was compared.