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International Journal of Genomics
Volume 2013, Article ID 629543, 16 pages
http://dx.doi.org/10.1155/2013/629543
Research Article

Comparative Study of Genome Divergence in Salmonids with Various Rates of Genetic Isolation

1Belozersky Institute for Physico-Chemical Biology of Lomonosov Moscow State University, Leninskie gory, 1, Moscow 119991, Russia
2Biological Department of Lomonosov Moscow State University, Leninskie Gory 1, Moscow 119991, Russia
3Russian Federative Research Institute of Fisheries and Oceanology, 17A V. Krasnoselskaya Street, Moscow 107140, Russia

Received 15 October 2012; Revised 22 March 2013; Accepted 15 May 2013

Academic Editor: Dmitry Sherbakov

Copyright © 2013 Elena A. Shubina et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of the study is a comparative investigation of changes that certain genome parts undergo during speciation. The research was focused on divergence of coding and noncoding sequences in different groups of salmonid fishes of the Salmonidae (Salmo, Parasalmo, Oncorhynchus, and Salvelinus genera) and the Coregonidae families under different levels of reproductive isolation. Two basic approaches were used: (1) PCR-RAPD with a 20–22 nt primer design with subsequent cloning and sequencing of the products and (2) a modified endonuclease restriction analysis. The restriction fragments were shown with sequencing to represent satellite DNA. Effects of speciation are found in repetitive sequences. The revelation of expressed sequences in the majority of the employed anonymous loci allows for assuming the adaptive selection during allopatric speciation in isolated char forms.