International Journal of Genomics

Research Article

Comparative Transcriptional Profiling of Two Contrasting Barley Genotypes under Salinity Stress during the Seedling Stage

Table 7

The primers used for real-time PCR in this study.


Probe ID	Primer sequences (5′-3′)	Annotations	Fold change
Probe ID	Primer sequences (5′-3′)	Annotations	Hua 30	Hua 11

Contig5422_at	F: CAATCCTCCAACACCAGTCA R: TCGGCAGGGACATCAAAG	Brassinosteroid insensitive 1-associated receptor kinase 1 precursor	0.8	1.15
Contig5740_at	F: GAGAATGGTAACGAGGTGG R: GGAGTTGTGTGAGCAGGGA	NAC domain-containing protein	0.15	1.15
Contig6159_at	F: CCTTCTTTCGTTTCTGCT R: GGCACTATTTCCATTACCT	PTF1	0.55	1.5
Contig7044_at	F: ACCCTCTACCACAACCGCCTCT R: ACCAACGACAGCCGCAGCA	Lung seven transmembrane receptor domain containing protein	0.05	1
Contig10690_at	F: ATCGTGTCCTCGTCGTCTTC R: AGTAGGTGGTTGCTGCTC	BZip type transcription factor bZIP1	0.15	1.55
Contig20719_at	F: ACGACGAGGACAGCGGCAACTT R: TTCACCTTGACGCCTTCTCCAC	Receptor-like protein kinase 5 precursor	0.7	4.3
HB03A08_T3_at	F: CAGATGATGGCGTTCCTCG R: TGCTCTGTGCTGCCTCCT	HSF-type DNA-binding domain containing protein	3.3	5.55

Contig13791_at	F: TCTTGGGTGATGAATGTGGTG R: AGGTTCGTGTTCCATCTC	Methyltransferase	0.55	1.2
Contig24300_at	F: ACAAACAGCATCCGAGCA R: GCCTCCAAAGTTCACATCCT	Cytokinin dehydrogenase precursor	0.9	2.4
HC02E04_T3_at	F: GAAGAAGGCGGTATGCGT R: CAGTACTTGTGAGGTAGTAGAAC	No hits	2.1	6.35

Ten salt-responsive genes in this list were selected randomly based on the microarray. The first seven pairs of primers belong to the shoot tissue, and the remaining belong to the root tissue. F: forward primer. R: reverse primer.