Research Article

Transcriptome of the Deep-Sea Black Scabbardfish, Aphanopus carbo (Perciformes: Trichiuridae): Tissue-Specific Expression Patterns and Candidate Genes Associated to Depth Adaptation

Table 7

Comparison of protein sequences of depth related genes between Aphanopus carbo database (All_gs454_xxx) and other fishes. Diff.: amino acid differences; Id. %: percentage of identity; Gaps: number of gaps introduced in the complete alignment and Acc. nr.: NCBI Accession number. 1Partial sequence; 2only AA sequence available.
(a) LDH-A (332 AA)

All_gs454_00149 vs. Diff.Id. %GapsAcc. nr.

Sphyraena idiastes 1695.180U80001
Rhinogobiops nicholsii 1794.880AF079534
Chromis caudalis 2193.670AY289558
Chionodraco rastrospinosus 2692.171AF079829
Notothenia coriiceps 2791.871AF079822
Fundulus heteroclitus 2791.870L43525
Cyprinus carpio 4486.750AF076528

(b) MDHc-A (333 AA)

All_gs454_00146 vs. Diff.Id. %GapsAcc. nr.

Oryzias latipes 1595.500NM_1163134
Sphyraena idiastes 2093.990AF390559
Osmerus mordax 3090.990BT075651
Salmo salar 3589.490BT060183

(c) Actin-1 (375 AA)

All_gs454_00104 vs. Diff.Id. %GapsAcc. nr.

Scomber scombrus 1 01000EF607093
Iniperca chuatsi 1 01000AY395872
Oryzias latipes 1 199.730NM_1104806
Pleurogrammus azonus 1 199.730AB073381
Coryphaenoides acrolepis 1 199.730AB021649
Coryphaenoides cinereus 1 199.730AB021651
Coryphaenoides armatus 2a 299.470AB086240
Coryphaenoides yaquinae 2a 299.470AB086242
Coryphaenoides acrolepis 2a 299.470AB021650
Coryphaenoides cinereus 2a 299.470AB021652
Cyprinus carpio 1 299.470AY395870
Sphyraena idiastes 1 399.200AF503593
Coryphaenoides armatus 2b 598.670AB086241
Coryphaenoides yaquinae 2b 598.670AB086243
Aphanopus carbo 2a 698.400All_gs454_00102
Sparus aurata 1 997.600AF190473

(d) LDH-B (334 AA)

All_gs454_00143 vs. Diff.Id. %GapsAcc. nr.

Lates calcarifer 1495.810FJ439507
Fundulus heteroclitus 2193.710L23792
Trachyrincus murrayi 4785.930AJ609235
Coryphaenoides armatus 5085.030AJ609232
Merlangius merlangus 5284.431AJ609234
Gadus morhua 5583.531AJ609233

(e) MyHC1 (305 AA)

All_gs454_00001 vs. Diff.Id. %GapsAcc. nr.

Paracirrhites forsteri 5394.950AJ243770
Seriola dumerilii 5494.860AB032020
Siniperca chuatsi 5994.380AY454304
Oryzias latipes 6294.102XM_4071618
Cyprinus carpio 6993.432D89992
Coryphaenoides acrolepis 8691.821AB330141
Notothenia coriiceps 8691.821AJ243767
Coryphaenoides yaquinae 8991.531AB330139
Coryphaenoides armatus 9291.251AB330140

(f) Hb-A1 (143 AA)

All_gs454_01074 vs. Diff.Id. %GapsAcc. nr.

Notothenia angustata 3972.731P623632
Boreogadus saida 4369.930DQ125471
Arctogadus glacialis 4469.930DQ125475
Takifugu rubripes 4667.830XM_3964767
Gadus morhua 5362.940O424252

(g) Hb-B1 (146 AA)

All_gs454_01018 vs. Diff.Id. %GapsAcc. nr.

Epinephelus coioides 2781.630GU982530
Anoplopoma fimbria 3178.910BT082849
Gasterosteus aculeatus 3178.910NM_1267638
Boreogadus saida 4072.790Q1AGS62
Notothenia angustata 4271.430P296282