Genetic Polymorphisms of Interleukin-1 Alpha and the Vitamin D Receptor in Mexican Mestizo Patients with Intervertebral Disc Degeneration

Table 1

Conditions and products of PCR-RFLP.

Gene	SNPs	Primers	Tm	RE	bp	Alleles
			(°C)			(bp)
IL1A	rs1800587	5′-GCATGCCATCACACCTAGTT-3′	56	NcoI	193	C: 174 and 19
		5′-TTACATATGAGCCTTCCATG-3′				T: 193
VDR	rs2228570	5′-AGCTGGCCCTGGCACTGCTCTGCTCT-3′	60	FokI	265	f: 197 and 68
		5′-ATGGAAACACCTTGCTTCTTCTCCCTC-3′				F: 265
	rs731236	5′-CAGAGCATGGACAGGGAGCAAG-3′	59	I	746	T: 495 and 251
		5′-GCAACTCCTCATGGCTGAGGTCTCA-3′				t: 294, 251, and 201

Tm: annealing temperature; RE: restriction enzyme.