High Expression of Polo-Like Kinase 1 Is Associated with Early Development of Hepatocellular Carcinoma
Figure 4
Knockdown of PLK1 in HCC accelerates cell apoptosis. BCL-7402 cells were transfected with PLK1 siRNA. 48 h later, (a) cells were harvested and subjected to annexin V and PI staining for apoptosis analysis by flow cytometry; (b) cells were observed using transmission electronic microscope (TEM); (c) cells were analyzed for caspase 3, caspase 8, and caspase 9 mRNA expression by real-time PCR; (d) cells were analyzed for Bcl-2 and Bax mRNA expression by real-time PCR. Results are shown as mean ± SD. Data are representative of at least 3 independent experiments. ; .
