Figure 2: Quantification of expression levels of Igf2 variants and Igf2as transcripts in different tissues. Igf2 variants and Igf2as were quantified in muscle (a), liver (b), and brain (c) tissues of fetus, newborn (NB), and adult and included embryonic brain. V1, V2, V3, and Igf2as stand for Igf2 variants 1, 2, 3, and Igf2 antisense, respectively. The results are expressed in relative expression, calculated relative to the first value. Each bar represents the mean of three samples each analyzed in triplicate. The standard deviations are indicated as error bars. , , and .