Figure 4: Quantification of Igf2 variants expression levels in wild-type and DMR1-U2 knockout placenta. Quantification of Igf2 transcripts performed in wild-type (WT) and knockout (KO) placentas at different embryonic stages (E12, E14, E16, and E19). Igf2 variants 1 (a), 2 (b), and 3 (c) were measured by qPCR. The results are expressed in relative expression. Each bar represents the mean of three samples each quantified in triplicate. The standard deviation is indicated by error bars. .