Gene Expression Profiling of Grass Carp (Ctenopharyngodon idellus) and Crisp Grass Carp

Figure 3

Quantitative real-time PCR confirmation of six differentially expressed genes identified by microarray analysis in crisp grass carp (CGC) versus grass carp (GC). Three samples were used for quantitative real-time PCR confirmation for experimental group and control group. β-Actin gene was used as an internal control. HSP70 for heat shock cognate 70-kd protein, HSP90 for heat shock protein 90 kDa alpha (cytosolic, B1), MSTN for myostatin, COL1A1 for type I collagen (alpha-1), COL1A2 for type I collagen (alpha-2), and CALR for calreticulin. Differential expression was determined by one-way ANOVA ().