Research Article
Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants
Table 2
Details of cross transferability of 35 EST-SSR markers in eleven plants belonging to five different families.
| | Serial number | Amplification range | Datura metel | Datura innoxia | Withania coagulans | Withania somnifera | Capsicum annuum | Eclipta alba | Stevia rebaudiana | Citrullus colocynthis | Ocimum sanctum | Catharanthus roseus | Moringa oleifera | Primer transferability |
| | EMS-1 | 104–891 | 8 | 3 | 4 | 3 | 4 | 2 | 0 | 3 | 1 | 2 | 1 | 90.91 | | EMS-2 | 129–884 | 0 | 8 | 7 | 9 | 7 | 6 | 0 | 3 | 6 | 3 | 4 | 81.82 | | EMS-3 | 117–794 | 6 | 4 | 2 | 2 | 1 | 4 | 1 | 3 | 1 | 1 | 2 | 100.00 | | EMS-4 | 92–358 | 2 | 2 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 100.00 | | EMS-5 | 135–802 | 3 | 4 | 4 | 2 | 3 | 2 | 1 | 1 | 1 | 1 | 2 | 100.00 | | EMS-6 | 94–164 | 2 | 2 | 2 | 0 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 90.90 | | EMS-7 | 105–919 | 1 | 2 | 9 | 2 | 5 | 7 | 1 | 2 | 1 | 8 | 2 | 100.00 | | EMS-8 | 98–680 | 6 | 2 | 2 | 1 | 3 | 1 | 1 | 2 | 1 | 4 | 1 | 100.00 | | EMS-9 | 130–717 | 3 | 2 | 5 | 3 | 2 | 1 | 3 | 1 | 2 | 2 | 1 | 100.00 | | EMS-10 | 92–913 | 5 | 2 | 3 | 2 | 2 | 1 | 1 | 2 | 2 | 1 | 1 | 100.00 | | EMS-11 | 135–724 | 5 | 3 | 5 | 4 | 5 | 0 | 1 | 4 | 1 | 1 | 2 | 90.90 | | EMS-12 | 121–881 | 7 | 6 | 3 | 11 | 4 | 3 | 2 | 8 | 5 | 5 | 7 | 100.00 | | EMS-13 | 75–629 | 0 | 7 | 8 | 9 | 2 | 7 | 2 | 5 | 2 | 6 | 0 | 81.81 | | EMS-14 | 106–649 | 3 | 2 | 2 | 3 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 63.64 | | EMS-15 | 99–517 | 5 | 3 | 0 | 2 | 2 | 1 | 1 | 5 | 1 | 3 | 2 | 90.90 | | EMS-16 | 105–853 | 5 | 4 | 2 | 6 | 3 | 5 | 2 | 1 | 2 | 3 | 1 | 100.00 | | EMS-17 | 77–930 | 4 | 3 | 7 | 5 | 6 | 3 | 2 | 2 | 1 | 1 | 1 | 100.00 | | EMS-18 | 83–787 | 1 | 2 | 4 | 2 | 3 | 0 | 1 | 2 | 1 | 1 | 4 | 90.90 | | EMS-19 | 111–603 | 3 | 2 | 9 | 7 | 2 | 2 | 1 | 4 | 1 | 3 | 3 | 100.00 | | EMS-20 | 117–560 | 5 | 4 | 10 | 4 | 6 | 1 | 1 | 3 | 2 | 4 | 1 | 100.00 | | EMS-21 | 101–863 | 6 | 5 | 6 | 5 | 4 | 1 | 1 | 2 | 1 | 2 | 1 | 100.00 | | EMS-22 | 108–774 | 3 | 6 | 7 | 5 | 6 | 2 | 0 | 1 | 2 | 6 | 7 | 90.90 | | EMS-23 | 101–837 | 0 | 3 | 6 | 6 | 10 | 1 | 1 | 7 | 3 | 3 | 8 | 90.90 | | EMS-24 | 61–785 | 2 | 4 | 3 | 5 | 5 | 1 | 0 | 5 | 6 | 5 | 5 | 90.90 | | EMS-25 | 124–790 | 5 | 7 | 9 | 7 | 5 | 1 | 2 | 0 | 3 | 2 | 6 | 90.90 | | EMS-26 | 50–425 | 7 | 3 | 3 | 4 | 4 | 1 | 1 | 1 | 1 | 2 | 1 | 100.00 | | EMS-27 | 124–564 | 5 | 4 | 4 | 4 | 0 | 3 | 0 | 4 | 1 | 1 | 1 | 81.81 | | EMS-28 | 50–1000 | 4 | 4 | 5 | 6 | 2 | 2 | 3 | 2 | 3 | 4 | 9 | 100.00 | | EMS-29 | 91–504 | 2 | 2 | 4 | 6 | 10 | 4 | 2 | 1 | 2 | 1 | 1 | 100.00 | | EMS-30 | 139–900 | 8 | 7 | 6 | 7 | 4 | 4 | 0 | 2 | 6 | 6 | 5 | 90.90 | | EMS-31 | 97–388 | 4 | 1 | 2 | 0 | 2 | 2 | 0 | 1 | 1 | 2 | 3 | 81.81 | | EMS-32 | 84–774 | 2 | 4 | 5 | 7 | 5 | 1 | 0 | 2 | 1 | 0 | 2 | 81.81 | | EMS-33 | 119–419 | 4 | 1 | 5 | 2 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 100.00 | | EMS-34 | 117–795 | 5 | 5 | 11 | 5 | 7 | 1 | 1 | 2 | 1 | 2 | 1 | 100.00 | | EMS-35 | 127–951 | 3 | 3 | 3 | 4 | 3 | 3 | 0 | 0 | 2 | 2 | 2 | 81.81 | | | | 134 | 126 | 150 | 152 | 132 | 77 | 54 | 84 | 67 | 91 | 90 | | | Percent of transferability of each plant | 86.45 | 81.29 | 96.77 | 98.06 | 85.16 | 49.68 | 34.84 | 54.19 | 43.23 | 58.71 | 58.06 | 67.86% |
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