Changes in Oleic Acid Content of Transgenic Soybeans by Antisense RNA Mediated Posttranscriptional Gene Silencing
Figure 1
Agrobacterium-mediated soybean transformation using the cotyledonary node as explants. (a) Seed germination in the GM medium for the night. (b) Inoculation of explants with Agrobacterium. (c) A longitudinal cut between the cotyledons and through the hypocotyl to generate two identical explants. (c) Explants cocultured with Agrobacterium for 5 days. (d) Two weeks after selection on shoot induction (SI) medium containing 5 mg/L glufosinate. (e) Elongated shoot in shoot elongation (SE) medium. (f) Rooting of the resistant shoot.
