Changes in Oleic Acid Content of Transgenic Soybeans by Antisense RNA Mediated Posttranscriptional Gene Silencing

Figure 3

Procedure for confirmation of transgenic plants. (a) Identification of the herbicide resistance of the leaves of transgenic soybean plants using 135 mg/L Basta. Left: negative transgenic plant; right: positive transgenic plants. (b) Events of the PCR positive lines were randomly selected and tested using LibertyLink strips. (c) PCR analysis of genome DNA of putative transgenic soybean plant. The length of PCR production was 752 bp. M: trans2K plus DNA marker; +: plasmid DNA; −: nontransformed soybean; H₂O. 1–14: the transgene line.

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