Genomic Changes in an Attenuated ZB Strain of Foot-and-Mouth Disease Virus Serotype Asia1 and Comparison with Its Virulent Parental Strain

Table 1

Primers used for amplification of the FMDV ZB strain genome.

Primer	Sequence (5′-3′)	Fragment	Coordinates^a
F1	TTGAAAGGGGGCGCTAGGTCT	F1	1–22
R1011	CCTATTCAGGCGTAGAAGCTT	F1	991–1011
F916	CACTGGTGACAGGCTAAGGATG	F2	887–908
R2383	CCGTCATGTTGGTGCGTGGGTT	F2	2363–2384
F1608	ACGATCAGGAACCACTCAACG	F3	1608–1628
R4100	AGCTTGTACCAGGGTTTGGC	F3	4081–4100
F3970	CAGATGCAGGAGGACATGTC	F4	3970–3989
R6434	AGAGGCCAGGCATGGTGTC	F4	6406–6424
F5404	GAAAGGCCAACACGAAGCAGC	F5	5373–5393
R6944	TCCATGGCGTCAAGTCCGTCGACGC	F5	6920–6944
F6610	GGGTTGATCGTTGACACCAGAGA	F6	6610–6632
RES-dT18	GCGGCCGCGATATCT18	F6	Poly A

Nucleotides position corresponds to the nucleotide sequence of the ZB/CHA/58(att) (GenBank number DQ533483).