Genomic Changes in an Attenuated ZB Strain of Foot-and-Mouth Disease Virus Serotype Asia1 and Comparison with Its Virulent Parental Strain

Table 3

Comparison of amino acid differences in the protein coding region between the virulent and attenuated ZB strains.

Region	Position*	ZBCF22	ZBRF168	ZBRF188	ZB/CHA/58(att)	YNBS/58	Corresponding residue in ref.^†
L^pro	2	N	D	D	D	N	N
	143	M	L	L	L	M	M
	147	E	G	G	G	E	E
VP2	133	D	D	D	G	D	D
	184	V	L	L	L	V	V
VP1	4	A	T	T	T	A	A/T
	21	A	E	E	E	A	E
	45	P	L	L	L	P	P
	81	A	V	V	V	A	V
	142	S	R	R	R	S	M/R
	146	P	P	P	F	P	R/L/M
	147	S	A	A	A	S	A
	155	G	G	G	R	G	K/N/G/A
2A	8	K	K	K	E	K	K
2B	19	N	S	S	S	N	N
	74	A	T	T	T	A	A
	136	V	I	I	I	V	V
2C	6	V	I	I	I	V	I
	64	K	E	E	E	K	K
	135	T	T	I	I	T	T
	248	I	T	T	T	T	I/L
	313	Y	H	H	H	Y	H
3A	51	A	A	A	G	A	A
	78	E	G	G	G	E	E
	80	H	C	C	C	H	R
	84	K	N	N	N	K	K/Q
	97	A	P	P	P	A	A
	107	D	D	N	N	D	D
	127	R	I	I	I	R	R
3C^pro	74	V	I	I	I	V	I
3D^pol	84	R	H	H	H	R	R
	158	V	A	A	A	V	A/V
	378	H	Q	Q	Q	H	H

The amino acids listed are at equivalent positions of ZB/CHA/58(att) (GenBank number DQ533483).
^†The FMDV serotype Asia1 reference strains are YNBS/58(AY390432), PAK/54(AY593795), IND63/72(304994), IND 321/01(AY687333), IND 491/97(AY687334), ISRL13/63(AY593796), Asia1/3 Kimorn(AY593797), and Leb 3/83(AY593798).