Research Article
Quantification and Gene Expression Analysis of Histone Deacetylases in Common Bean during Rust Fungal Inoculation
Figure 4
PCR amplification of HDAC genes under study. (a) Primers for the selected genes were amplified by PCR with Sierra genomic DNA and electrophoresed on a 2% agarose gel. In positive (+ve) control, reference gene TC362 primers were used and in negative (−ve) control no primers were added to the PCR reaction. (b) The same HDACs primers were tested with experimental cDNA by PCR amplification and electrophoresed on a 2% agarose gel. In +ve control, genomic DNA was used instead of cDNA and in −ve control no primers were added to the PCR reaction.
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