Identification of Immune Related LRR-Containing Genes in Maize (Zea mays L.) by Genome-Wide Sequence Analysis

Figure 3

Unrooted phylogenetic tree of the seven identified LRR-RLKs showing the relationship with their homologues in other plants. This tree was constructed from a ClustalW generated multiple sequence alignment of amino acid sequences using the neighbor-joining method in MEGA5. Node values indicate percentage bootstrap values derived from 10,000 replications. The maize LRR-RLKs reported in this study are shaded in grey. The accession numbers of the sequences used for the phylogenetic analysis are as follows: Zea mays PSY1R [XP 008644981], PSKR1 [XP 008645956], BIR1 [NP 001147794], OsSERK1 [XP 008678722], Cf5 [NP 001132758], and FLS2 [XP 008668880]; Sorghum bicolor PSY1R [XP 002437497], PSKR1 [XP 002454207], BIR1 [XP 002450551], Cf5 [XP 002465654], and FLS2 [Sb06g028760]; Setaria italica PSY1R [XP 004966176] and Cf5 [XP 004985227]; Oryza sativa Indica Group PSY1R [AAU12600] and FLS2 [CAH68341]; Oryza sativa Japonica Group PSKR1 [BAD23737], OsSERK1 [AAU88198], Cf5 [NP 001049352], and FSL2 [CAE02151]; Brachypodium distachyon Cf5 [XP 003558509] and FLS2 [BRADI5G21960]; Triticum aestivum FLS2 [6E8764762]; Triticum urartu FLS2 [EMS63184]; Arabidopsis thaliana PSY1R [NP 177374], PSKR1 [NP 178330], BIR1 [NP 568696], Cf5 [NP 200932], and FLS2 [NP 199445]; Brassica rapa PSY1R [XP 009105890] and PSKR1 [XP 009129163]; Glycine max PSY1R [XP 003520891]; Nicotiana sylvestris PSY1R [XP 009759322] and FLS2 [XP 009801818]; Morus notabilis PSKR1 [EXC37937]; Vitis vinifera PSKR1 [XP 002273186] and OsSERK1 [XP 002270847]; Solanum tuberosum OsSERK1 [NP 001275293].