RNA Degradation in Staphylococcus aureus: Diversity of Ribonucleases and Their Impact

Figure 3

SAOUHSC_01913, a putative pyrophosphohydrolase in S. aureus. (a) Protein sequence alignment of RppH_Bs from B. subtilis AG1839 (Genbank accession number CP008698.1) and SAOUHSC_01913 (RppH_Sa) from S. aureus NCTC8325 (Genbank accession number NC_007795). The NUDIX motif is underlined and the conserved amino acid residues within the NUDIX region are in bold. Identical residues between both proteins are indicated by a star. (b) Artemis visualization of a randomly chosen region within the chromosome of HG003. The bottom part represents the six open reading frames, indicated by blue arrows. The upper part represents strand coverage of HG003 in red and HG003 rppH in blue. No coverage difference is observed between the two strains. (c) Volcano plot representation (a scatter-plot constructed by plotting the negative log of the value on the -axis and the log of the fold change between the two conditions on the -axis) of DEseq analysis between HG003 and HG003 rppH. The red dots correspond to mRNAs differentially expressed according to the fold change and the adjusted value (fold change of 1.5 and a value > 0.05). The most differentially expressed RNA corresponds to rppH mRNA itself as indicated by the red circle.

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