Characterization and Development of EST-SSRs by Deep Transcriptome Sequencing in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

Figure 5

PCR products amplified by nineteen effective EST-SSR primer pairs in twenty-four cultivars of Chinese cabbage. The order of DNA samples from lane 1 to lane 24 within each primer pair image panel is 682, GuangDongZao, ZaoHuangBai, Z61-8, FuShanBaoTou, Li-3, 212-7, TianJinQingMaYe, KuaiCai number 6-5, JinHuangXiaoBaiCai, SiJiXiaoBaiCai, SiJiHuangYangXiaoBaiCai, PinZao number 1, HanYuTeXuanHuangXin, QuanNengSiJiKuaiCai, JingYouXiaoBaiCaiKuaiCai, GaoLiWaWaCai, KeYiXiaWaWa, JinNuoChunQiuWaWaCai, SiJiLvGanXiaoKuCai, YouLv157, ShuYaoYouCai, DeGaoYouLiangQingGengCai, and QingXiuF1QingGengCai. PCR products amplified by BR-es2, BR-es3, BR-es6, BR-es7, BR-es12, BR-es13, BR-es14, BR-es16, and BR-es19 primer pairs were separated on 6% denaturing polyacrylamide gels, while those amplified by BR-es1, BR-es4, BR-es5, BR-es8, BR-es9, BR-es10, BR-es11, BR-es15, BR-es17, and BR-es18 primer pairs were separated on 12% nondenaturing polyacrylamide gels.